Crassaminicella thermophila sp. nov., a moderately thermophilic bacterium isolated from a deep-sea hydrothermal vent chimney and emended description of the genus Crassaminicella
Crassaminicella thermophila sp. nov., a moderately thermophilic bacterium isolated from a deep-sea hydrothermal vent chimney and emended description of the genus Crassaminicella
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嗜热克拉萨米菌
DOI：
10.1099/ijsem.0.005112
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发表时间：
2021
影响因子：
2.8
通讯作者：
Wu Long Fei
中科院分区：
文献类型：
--
作者：
Li Xue Gong;Lin Jin;Bai Shi Jie;Dai Jie;Jiao Ze Xi;Tang Hong Zhi;Qi Xiao Qing;Zhang Wei Jia;Liu Min;Xu Jian Sheng;Wu Long Fei
从位于西南印度洋脊2730 m深处的热液喷口烟囱中分离到一株新的中度嗜热、厌氧、异养细菌（菌株SY 095 T）。细胞为革兰氏染色阳性，能动，直至略弯曲的杆状，形成末端内生孢子。SY 095 T在45-60 °C（最佳50-55 °C）、pH 6.0-7.5（最佳7.0）和1- 4.5%（w/v）NaCl（最佳2.5%）的盐度下生长。 SY 095 T利用的底物包括果糖、葡萄糖、麦芽糖、N-乙酰氨基葡萄糖和胰蛋白胨。还利用酪蛋白氨基酸和氨基酸（谷氨酸、谷氨酰胺、赖氨酸、甲硫氨酸、丝氨酸和组氨酸）。葡萄糖发酵的主要最终产物是醋酸盐、H2和CO2。元素硫、硫酸盐、硫代硫酸盐、亚硫酸盐、富马酸盐、硝酸盐、亚硝酸盐和Fe（III）不用作末端电子受体。主要的细胞脂肪酸是C14：0（60.5%）和C16：0（7.6%）。 主要极性脂质包括二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、五种未鉴定的磷脂和两种未鉴定的氨基磷脂。未检测到呼吸醌类。染色体DNA G+C含量为30.8mol%。系统发育分析表明，SY 095 T与Crassaminicella profundaRa 1766 HT的16 S rRNA基因同源性为95.8%。 SY 095与C. profundaRa1766HT.计算机DNA-DNA杂交（DDH）结果表明，SY 095 T与C. profundaRa1766HT.根据SY 095 T的表型、基因型和系统发育特征，认为它代表了Crassaminicella属的一个新种，并将其命名为Crassaminicella thermophilasp。十一是建议。模式菌株为SY 095 T（=JCM 34213=MCCC 1 K 04191）。并提出了厚壳菌属的订正描述。
A novel moderately thermophilic, anaerobic, heterotrophic bacterium (strain SY095T) was isolated from a hydrothermal vent chimney located on the Southwest Indian Ridge at a depth of 2730 m. Cells were Gram-stain-positive, motile, straight to slightly curved rods forming terminal endospores. SY095Twas grown at 45–60 °C (optimum 50–55 °C), pH 6.0–7.5 (optimum 7.0), and in a salinity of 1–4.5 % (w/v) NaCl (optimum 2.5 %). Substrates utilized by SY095Tincluded fructose, glucose, maltose,N-acetyl glucosamine and tryptone. Casamino acid and amino acids (glutamate, glutamine, lysine, methionine, serine and histidine) were also utilized. The main end products from glucose fermentation were acetate, H2and CO2. Elemental sulphur, sulphate, thiosulphate, sulphite, fumarate, nitrate, nitrite and Fe(III) were not used as terminal electron acceptors. The predominant cellular fatty acids were C14 : 0(60.5%) and C16 : 0(7.6 %). The main polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, five unidentified phospholipids and two unidentified aminophospholipids. No respiratory quinones were detected. The chromosomal DNA G+C content was 30.8 mol%. The results of phylogenetic analysis of the 16S rRNA gene sequences indicated that SY095Twas closely related toCrassaminicella profundaRa1766HT(95.8 % 16S rRNA gene sequence identity). SY095Texhibited 78.1 % average nucleotide identity (ANI) toC. profundaRa1766HT. Thein silicoDNA–DNA hybridization (DDH) value indicated that SY095Tshared 22.7 % DNA relatedness withC. profundaRa1766HT. On the basis of its phenotypic, genotypic and phylogenetic characteristics, SY095Tis suggested to represent a novel species of the genusCrassaminicella, for which the nameCrassaminicella thermophilasp. nov. is proposed. The type strain is SY095T(=JCM 34213=MCCC 1K04191). An emended description of the genusCrassaminicellais also proposed.