Interleukin-1 receptor antagonist gene VNTR polymorphism is associated with coronary artery disease
Interleukin-1 receptor antagonist gene VNTR polymorphism is associated with coronary artery disease
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DOI：
10.1590/s0066-782x2008001700002
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发表时间：
2008-11-01
影响因子：
2.6
通讯作者：
Tezel, Tuna
中科院分区：
文献类型：
--
作者：
Arman, Ahmet;Soylu, Ozer;Tezel, Tuna
背景：冠状动脉疾病（CAD）是向心肌输送血液的冠状动脉的动脉粥样硬化。动脉粥样硬化是一种炎症性疾病。与IL1家族相关的细胞因子基因变异参与动脉粥样硬化的发病机制。 目的：本研究的目的是确定土耳其人群中IL1家族多态性（IL1RN VNTR、IL1B位置-511和+3953）与CAD之间的关系。 方法：427名个体接受冠状动脉造影，分为170名对照受试者和257名CAD受试者患者。 CAD 受试者分为两个亚组：91 名单血管疾病 (SVD) 受试者和 166 名多血管疾病 (MVD) 受试者。通过聚合酶链式反应 (PCR) 和限制性消化分析确定 IL1RN 和 IL1B (-511, +3953) 的基因型。 结果：CAD 和对照受试者或 MVD 和对照受试者之间 IL1RN 和 IL1B (-511 和 +3953) 基因型分布没有发现显着差异。然而，SVD 和对照受试者之间的 IL1RN 2/2 基因型存在显着相关性（P = 0.016，x2：10.289，OR：2.94，95% CI：1.183-7.229）。同样，CAD 和对照受试者、MVD 和对照受试者或 SVD 和对照受试者之间的 IL1RN 和 IL1B（-511 和 +3953）等位基因频率没有发现统计学显着差异。结论：CAD 和对照组之间的 IL1RN 和 IL1B 多态性等位基因频率或基因型分布没有发现关联。然而; IL1RN 2/2 基因型可能是土耳其人群发生 SVD 的危险因素。
BACKGROUND: Coronary Artery Disease (CAD) is the atherosclerosis of coronary arteries that carry blood to the heart muscle. Atherosclerosis is an inflammatory disease. Cytokine gene variations such as those associated with the IL1 family are involved in the pathogenesis of atherosclerosis.OBJECTIVE: The purpose of this study was to determine the relationship between IL1 family polymorphisms (IL1RN VNTR, IL1B positions -511 and +3953) and CAD in Turkish population.METHODS: 427 individuals were submitted to coronary angiography and were grouped as 170 control subjects and 257 CAD patients. The CAD subjects were divided into two subgroups: 91 Single Vessel Disease (SVD) and 166 Multiple Vessel Disease (MVD) subjects. The genotypes of IL1RN and of IL1B (-511, +3953) were determined by polymerase chain reaction (PCR) followed by restriction digestion analysis.RESULTS: No significant difference was found in IL1RN and IL1B (-511 and +3953) genotype distributions between CAD and control subjects or MVD and control subjects. However, significant association was seen in IL1RN 2/2 genotype between SVD and control subjects (P= 0.016, x2: 10.289, OR: 2.94, 95% CI: 1.183-7.229). Similarly, no statistically significant difference was found in IL1RN and IL1B (-511 and +3953) allele frequencies between CAD and control subjects, MVD and control subjects or SVD and control subjects.CONCLUSION: No association was found in either allele frequency or genotype distribution of IL1RN and IL1B polymorphisms between CAD and the control groups. However; IL1RN 2/2 genotype may be a risk factor for SVD in the Turkish population.