Genetic and Pathobiologic Characterization of Pandemic H1N1 2009 Influenza Viruses from a Naturally Infected Swine Herd
Genetic and Pathobiologic Characterization of Pandemic H1N1 2009 Influenza Viruses from a Naturally Infected Swine Herd
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DOI：
10.1128/jvi.02118-09
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发表时间：
2010-03-01
影响因子：
5.4
通讯作者：
Pasick, John
中科院分区：
文献类型：
--
作者：
Weingartl, Hana M.;Berhane, Yohannes;Pasick, John
自从在墨西哥和美国首次发现这种新型H1N1流感病毒以来，人们一直担心，这种新型H1N1流感病毒可能会导致一场与1918年大流行相当的大流行。2009年4月下旬，从加拿大养猪场暴发的H1N1流感病毒中分离出与大流行性H1N1流感病毒系统发育相关的病毒。此次暴发还与一例疑似人间病例有流行病学联系。使用此次暴发的猪分离株之一和人分离株A/墨西哥/InDRE4487/2009对猪进行的实验性感染显示，病毒从下呼吸道恢复的情况有所不同。从感染A/墨西哥/InDRE4487/2009的猪的肺中始终分离到病毒，而感染A/Sine/Alberta/OTH-33-8/2008的猪中只有一头从肺中产生活病毒，尽管两组猪的病毒RNA和抗原数量相当。临床疾病类似于猪的其他流感病毒感染，尽管病毒抗原检测时间有所延长，病毒RNA从肺部清除的时间也有所延迟。从养猪场分离的病毒中也存在大量的基因变异。这一点，加上在实验感染的动物中观察到的有些不规则的病理生物学特征，表明尽管病毒可能来自猪，但重大的病毒进化可能仍在进行中。
Since its initial identification in Mexico and the United States, concerns have been raised that the novel H1N1 influenza virus might cause a pandemic of severity comparable to that of the 1918 pandemic. In late April 2009, viruses phylogenetically related to pandemic H1N1 influenza virus were isolated from an outbreak on a Canadian pig farm. This outbreak also had epidemiological links to a suspected human case. Experimental infections carried out in pigs using one of the swine isolates from this outbreak and the human isolate A/Mexico/InDRE4487/2009 showed differences in virus recovery from the lower respiratory tract. Virus was consistently isolated from the lungs of pigs infected with A/Mexico/InDRE4487/2009, while only one pig infected with A/swine/Alberta/OTH-33-8/2008 yielded live virus from the lung, despite comparable amounts of viral RNA and antigen in both groups of pigs. Clinical disease resembled other influenza virus infections in swine, albeit with somewhat prolonged virus antigen detection and delayed viral-RNA clearance from the lungs. There was also a noteworthy amount of genotypic variability among the viruses isolated from the pigs on the farm. This, along with the somewhat irregular pathobiological characteristics observed in experimentally infected animals, suggests that although the virus may be of swine origin, significant viral evolution may still be ongoing.