Shikonin blocks migration and invasion of human breast cancer cells through inhibition of matrix metalloproteinase-9 activation
Shikonin blocks migration and invasion of human breast cancer cells through inhibition of matrix metalloproteinase-9 activation
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DOI：
10.3892/or.2014.3159
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发表时间：
2014-06-01
期刊：
影响因子：
4.2
通讯作者：
Kim, Jong-Ho
中科院分区：
文献类型：
--
作者：
Jang, Soon Young;Lee, Jae Koan;Kim, Jong-Ho
紫草素是一种从传统中草药中分离的天然萘醌，有报道可促进肿瘤细胞死亡。然而，关于其对转移相关细胞侵袭和迁移行为的影响的报道很少。在本研究中，我们研究紫草素对人乳腺癌侵袭和迁移的影响。我们发现，紫草素抑制佛波酯12-肉豆蔻酸酯13-乙酸酯（PMA）诱导的MCF-7乳腺癌细胞的细胞迁移和侵袭，这是与调制基质金属蛋白酶-9（MMP-9）通过抑制表达和蛋白水解和启动子活性。我们还发现，紫草素抑制MMP-9的表达和启动子活性的MDA-MB-231细胞具有高转移潜力。这些结果表明紫草素通过调节MMP-9抑制人乳腺癌细胞的迁移和侵袭。因此，紫草素有可能成为一种潜在的抗乳腺癌药物。
Shikonin, a natural naphthoquinone isolated from a traditional Chinese medicinal herb, has been reported to promote tumor cell death. However, there are few reports concerning its effect on metastasis-related cell invasion and migration behavior. In the present study, we investigated the effect of shikonin on human breast cancer invasion and migration. We found that shikonin inhibited phorbol 12-myristate 13-acetate (PMA)-induced cell migration and invasion in MCF-7 breast cancer cells, which was correlated with modulation of matrix metalloproteinase-9 (MMP-9) through suppression of both expression and proteolytic and promoter activity. We also found that shikonin inhibited both MMP-9 expression and promoter activity in MDA-MB-231 cells with high metastatic potential. These results indicated that shikonin induces the suppression of migration and invasion through modulation of MMP-9 in human breast cancer cells. Therefore, shikonin may be a potential anticancer drug for human breast cancer therapy.