SIMPLE CHEMICALLY DEFINED MEDIUM FOR PRODUCTION OF PHASE-1 BORDETELLA-PERTUSSIS
SIMPLE CHEMICALLY DEFINED MEDIUM FOR PRODUCTION OF PHASE-1 BORDETELLA-PERTUSSIS
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DOI：
10.1099/00221287-63-2-211
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发表时间：
1970-01-01
期刊：
影响因子：
--
通讯作者：
SCHOLTE, MJ
中科院分区：
文献类型：
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作者：
STAINER, DW;SCHOLTE, MJ
本文介绍了一种简单的、化学成分确定的培养基，该培养基由谷氨酸钠、脯氨酸、胱氨酸、盐类和生长因子组成，适合于百日咳杆菌阶段的大规模生产，其菌数可达30 × 109个/ml以上。在48 ~ 72 h内产生。在摇瓶和发酵罐中生长。通过加入0.14%福尔马林对培养物进行解毒，以产生对小鼠和豚鼠无毒且具有良好小鼠保护性抗原水平的疫苗。最终疫苗在高温下储存后，抗原稳定性令人满意。
A simple, chemically defined medium is described consisting of sodium glutamate, proline, cystine, salts, and growth factors, which is suitable for the large-scale production of phase IBordetella pertussis.More than 30 × 109organisms/ml. were produced in 48 to 72 h. growth in shake flasks and fer- mentors. The cultures were detoxified by the addition of 0·14 % formalin to yield vaccines which were non-toxic to mice and guinea pigs and had good mouse-protective antigen levels. The antigenic stability was satisfactory after storage of the final vaccines at elevated temperatures.