Characterization of adult alpha- and beta-globin genes in the zebrafish
Characterization of adult alpha- and beta-globin genes in the zebrafish
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DOI：
10.1182/blood.v89.2.688
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发表时间：
1997-01-15
期刊：
影响因子：
20.3
通讯作者：
Zon, LI
中科院分区：
文献类型：
--
作者：
Chan, FY;Robinson, J;Zon, LI
大多数脊椎动物都会发生血红蛋白（Hbs）的发育转换，但这一过程的细胞和分子基础尚不清楚。斑马鱼是一种新的遗传和发育系统，可用于胚胎发生研究，近年来已衍生出具有多种造血缺陷的突变体。为了开始我们对这种生物体中Hb转换的研究，我们对成人中表达的珠蛋白进行了表征。成人外周血溶血物的反相高效液相色谱和质谱分析表明，循环红细胞中存在三种主要的α -球蛋白和两种β -球蛋白。此外，我们已经分离并鉴定了斑马鱼成年α -珠蛋白和β -珠蛋白cDNA克隆，这些克隆编码一些这些珠蛋白。在成年红细胞中检测到高水平的α -和β -珠蛋白基因表达，而胚胎红细胞表达很少，如果有的话。我们还表明，α -珠蛋白和β -珠蛋白基因在同一条染色体上紧密相连，分别以3‘-5’和5‘-3’的结构排列。这些基因的特征和调控元件在这个珠蛋白位点将提供深入了解珠蛋白基因的转录过程。有了这些试剂，未来对造血缺陷斑马鱼突变体中Hb转换的研究将成为可能。(C) 1997年由美国血液病学会出版。
Developmental switching of hemoglobins (Hbs) occurs in most vertebrates, yet the cellular and molecular basis far this process remains elusive. The zebrafish is a new genetic and developmental system that can be used to study embryogenesis, and mutants with a variety of defects in hematopoiesis have recently been derived. To initiate our studies on Hb switching in this organism, we have characterized the globins expressed in the adult. Reversed-phase high performance liquid chromatography and mass spectrometric analyses of adult peripheral blood hemolysates showed that there are three major alpha globins and two beta globins in circulating erythroid cells. In addition, we have isolated and characterized zebrafish adult alpha- and beta-globin cDNA crones that encode some of these globins. High levels of alpha- and beta-globin gene expression were detected in adult erythroid cells, whereas embryonic erythroid cells expressed little, if any, of these RNAs. We have also shown that the alpha- and beta-globin genes are tightly linked on the same chromosome and are arrayed in a 3'-5' to 5'-3' configuration, respectively. The characterization of these genes and regulatory elements in this globin locus will provide insight into the process of globin gene transcription. With these reagents, future studies of Hb switching in zebrafish mutants with defective hematopoiesis will be possible. (C) 1997 by The American Society of Hematology.