Biosorption and biotransformation of crystal violet by Aeromonas hydrophila DN322p
Biosorption and biotransformation of crystal violet by Aeromonas hydrophila DN322p
复制标题
DOI：
10.1007/s11783-012-0435-6
复制
发表时间：
2013-04
影响因子：
6.4
通讯作者：
T. Pan;Sui-zhou Ren;Jun Guo;Meiying Xu;G. Sun
中科院分区：
文献类型：
--
作者：
T. Pan;Sui-zhou Ren;Jun Guo;Meiying Xu;G. Sun
菌株DN 322 p是嗜水气单胞菌（AeromonasericaDN 322）的后代，具有同时吸附和脱色废水中三苯甲烷类染料的能力。选择常见的三苯甲烷染料结晶紫（CV）测试DN 322 p的脱色特性。在0.5 h内，菌株DN 322 p吸附大量CV，产生深色细胞沉淀和无色上清液。细胞沉淀和上清液的颜色随时间变浅。细胞沉淀的上清液和二氯甲烷提取物在紫外-可见光谱分析中分别在590 nm和260 nm处显示出明显的CV和隐色CV（LCV）特征吸收峰。这一发现表明，DN 322 p细胞可以吸附这两种染料。50 mg CV·L−1在30°C振荡2.5 h内达到99%（w/w）的脱色率。上清液和细胞沉淀的二氯甲烷提取物的高效液相色谱（HPLC）分析证实CV主要转化为其无色形式。死细胞具有与活细胞相似的吸附能力。在50 mg·L-1的染料溶液中，经高压灭菌的细胞可去除约90%的CV，其600 nm处的光密度（OD 600）大于1.0。
DN322p, an offspring ofAeromonas hydrophilaDN322, has the capacity to adsorb and decolorize triphenylmethane dyes in wastewater simultaneously. As a common triphenylmethane dye, crystal violet (CV) was chosen to test the decolorization characteristics of DN322p. Within 0.5 h, the strain DN322p adsorbed a large amount of CV, producing a deep-colored cell pellet and colorless supernatant. The colors of the cell pellet and supernatant lightened over time. The supernatant and dichloromethane extract of the cell pellet both showed conspicuous CV and leuco CV (LCV) characteristic absorbance peaks at 590 nm and 260 nm, respectively, in the UV-vis spectral analysis. This finding indicated that the DN322p cells can adsorb the two dyes. A 99% (w/w) decolorization rate was achieved within 2.5 h with shaking at 30°C for 50 mg CV·L−1. High Performance Liquid Chromatography (HPLC) analysis of the dichloromethane extract of the supernatant and cell pellet confirmed that CV was mainly converted into its leuco form. Dead cells had a similar adsorption capacity with living cells. About 90% of CV in the dye solution (50 mg·L−1) was removed by autoclaved cells with an optical delnsity at 600 nm (OD600) above 1.0.