U-shape suppressive effect of phenol red on the epileptiform burst activity via activation of estrogen receptors in primary hippocampal culture.
U-shape suppressive effect of phenol red on the epileptiform burst activity via activation of estrogen receptors in primary hippocampal culture.
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酚红通过激活原代海马培养物中雌激素受体对癫痫样爆发活动的 U 形抑制作用
DOI：
10.1371/journal.pone.0060189
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Liu X;Chen B;Chen L;Ren WT;Liu J;Wang G;Fan W;Wang X;Wang Y
酚红广泛用于细胞培养中作为pH指示剂。最近，它也被报道具有雌激素样生物活性，并能够促进不同细胞系的细胞增殖。然而，酚红对原代神经元培养的影响从未被研究过。应用膜片钳技术，我们证明了在不含酚红的神经基础培养基中培养的海马锥体神经元具有大的去极化相关癫痫样爆发活动，这在含酚红的培养基中培养的神经元中是罕见的。进一步的实验数据表明，酚红对异常癫痫样爆发神经元活动的抑制作用呈U形剂量相关，最有效浓度为28 μM。此外，雌激素受体激动剂17-β-雌二醇可模拟酚红对癫痫样神经元放电的浓度相关抑制作用，而雌激素受体拮抗剂ICI-182，780可抑制酚红对癫痫样神经元放电的浓度相关抑制作用。我们的研究结果表明，雌激素受体激活的酚红在培养基中防止形成异常，癫痫样爆发活动。这些研究强调了酚红作为雌激素受体刺激剂的重要性，并警告在细胞培养基中小心使用酚红。
Phenol red is widely used in cell culture as a pH indicator. Recently, it also has been reported to have estrogen-like bioactivity and be capable of promoting cell proliferation in different cell lines. However, the effect of phenol red on primary neuronal culture has never been investigated. By using patch clamp technique, we demonstrated that hippocampal pyramidal neurons cultured in neurobasal medium containing no phenol red had large depolarization-associated epileptiform bursting activities, which were rarely seen in neurons cultured in phenol red-containing medium. Further experiment data indicate that the suppressive effect of the phenol red on the abnormal epileptiform burst neuronal activities was U-shape dose related, with the most effective concentration at 28 µM. In addition, this concentration related inhibitory effect of phenol red on the epileptiform neuronal discharges was mimicked by 17-β-estradiol, an estrogen receptor agonist, and inhibited by ICI-182,780, an estrogen receptor antagonist. Our results suggest that estrogen receptor activation by phenol red in the culture medium prevents formation of abnormal, epileptiform burst activity. These studies highlight the importance of phenol red as estrogen receptor stimulator and cautions of careful use of phenol red in cell culture media.
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DOI：
10.1073/pnas.0912558106
发表时间：
2009-12-22
影响因子：
11.1
作者：
Zadran, Sohila;Qin, Qingyu;Baudry, Michel
通讯作者：
Baudry, Michel
影响因子：
3
作者：
Herzog, Andrew G.
通讯作者：
Herzog, Andrew G.
影响因子：
5.3
作者：
Veliskova, Jana;Velisek, Libor
通讯作者：
Velisek, Libor
影响因子：
3.7
作者：
Rao, ML;Kölsch, H
通讯作者：
Kölsch, H
BDNF-TrkB信号通路介导惊厥药物环噻嗪诱导的癫痫样活动
影响因子：
4.7
作者：
Wang, Yun;Qi, Jin-Shun;Kong, Shuzhen;Sun, Yajie;Fan, Jing;Jiang, Min;Chen, Gong
通讯作者：
Chen, Gong