Bursal transcriptome profiling of different inbred chicken lines reveals key differentially expressed genes at 3 days post-infection with very virulent infectious bursal disease virus
Bursal transcriptome profiling of different inbred chicken lines reveals key differentially expressed genes at 3 days post-infection with very virulent infectious bursal disease virus
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DOI：
10.1099/jgv.0.000956
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发表时间：
2018-01-01
影响因子：
3.8
通讯作者：
Omar, Abdul Rahman
中科院分区：
文献类型：
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作者：
Farhanah, Mohd Isa;Yasmin, Abd Rahaman;Omar, Abdul Rahman
传染性法氏囊病是家禽养殖业中的一种高度传染性疾病，会导致鸡的免疫抑制。超强毒传染性法氏囊病病毒(VvIBDV)感染后，不同遗传背景的近交鸡免疫应答基因的全基因组调控尚不清楚。因此，本研究旨在通过对6、7、15、N、O和P 6个近交系鸡感染vvIBDV UK661株后的法氏囊组织转录组进行分析，重点分析感染高峰期(感染后3天)感染鸡的重要基因和途径。所有感染的鸡都死于感染，不同品系之间没有重大差异。然而，根据病毒载量和法氏囊病变评分，P和6品系可被认为是最敏感的品系，而15和N品系被认为是受影响最小的品系。法氏囊转录组图谱鉴定出4588个差异表达基因，其中2985个基因表达上调，1642个基因表达下调，其中这些基因在所有或几个侵染品系中普遍或唯一检测到。上调的基因主要是促炎细胞因子、趋化因子和干扰素相关基因。与B细胞功能相关的各种基因和与凋亡相关的基因以及参与P53信号转导的基因都下调了。综上所述，不同自交系的法氏囊转录组图谱显示，在vvIBDV感染后，促炎症细胞因子和趋化因子、Th1细胞因子、JAK-STAT信号基因、MAPK信号基因及其相关途径的表达存在差异。
Infectious bursal disease is a highly contagious disease in the poultry industry and causes immunosuppression in chickens. Genome-wide regulations of immune response genes of inbred chickens with different genetic backgrounds, following very virulent infectious bursal disease virus (vvIBDV) infection are poorly characterized. Therefore, this study aims to analyse the bursal tissue transcriptome of six inbred chicken lines 6, 7, 15, N, O and P following infection with vvIBDV strain UK661 using strand-specific next-generation sequencing, by highlighting important genes and pathways involved in the infected chicken during peak infection at 3 days post-infection. All infected chickens succumbed to the infection without major variations among the different lines. However, based on the viral loads and bursal lesion scoring, lines P and 6 can be considered as the most susceptible lines, while lines 15 and N were regarded as the least affected lines. Transcriptome profiling of the bursa identified 4588 genes to be differentially expressed, with 2985 upregulated and 1642 downregulated genes, in which these genes were commonly or uniquely detected in all or several infected lines. Genes that were upregulated are primarily pro-inflammatory cytokines, chemokines and IFN-related. Various genes that are associated with B-cell functions and genes related to apoptosis were downregulated, together with the genes involved in p53 signalling. In conclusion, bursal transcriptome profiles of different inbred lines showed differential expressions of pro-inflammatory cytokines and chemokines, Th1 cytokines, JAK-STAT signalling genes, MAPK signalling genes, and their related pathways following vvIBDV infection.