Developmental evaluation of a potential non-steroidal estrogen: triclosan
Developmental evaluation of a potential non-steroidal estrogen: triclosan
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DOI：
10.1016/s0141-1136(00)00080-5
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发表时间：
2000-07-01
影响因子：
3.3
通讯作者：
Benson, WH
中科院分区：
文献类型：
--
作者：
Foran, CM;Bennett, ER;Benson, WH
三氯生是工业中常用的抗菌剂，经常在废水中检测到。由于三氯生的化学结构与已知的非甾体雌激素（例如DES、双酚A）非常相似，因此对三氯生作为内分泌干扰物的潜力进行了研究。日本青鳉鱼苗（Oryzias latipes）在孵化后2天开始暴露于三氯生（100、10、1 μ g/l）、17-β雌二醇（E2; 1 μ g/l）或溶剂对照（乙醇）14天。暴露后两个月，每只成虫的表型性别进行了评估视觉使用性二形鳍的形状和大小。三氯生暴露动物和溶剂处理对照组（乙醇53%、1 ppb 58%、10 ppb 45%、100 ppb 36%）中每组雌性动物的比例相似，尽管E2处理确实产生了92%的雌性成年动物。对性二形鳍性状进行了量化，以寻找三氯生和E2对第二性征发育的潜在影响。这些结果不支持三氯生具有强效雌激素性的假设。然而，鳍长度的变化和性别比例的非显著趋势表明三氯生具有潜在的弱雄激素性。(C)2000爱思唯尔科技有限公司版权所有。
Triclosan is an antibacterial agent commonly used in industry and often detected in wastewater effluent. The potential of triclosan to act as an endocrine disrupter was examined because its chemical structure closely resembles known non-steroidal estrogens (e.g. DES, bisphenol A). Japanese medaka fry (Oryzias latipes) were exposed for 14 days beginning 2 days post-hatch to triclosan (100, 10, 1 mug/l), 17-beta estradiol (E2; 1 mug/l), or a solvent control (ethanol). Two months post-exposure, the phenotypic sex of each adult was assessed visually using sexually dimorphic fin shape and size. The proportion of females in each group was similar for triclosan-exposed animals and solvent-treated controls (ethanol 53%, 1 ppb 58%, 10 ppb 45%, 100 ppb 36%) although E2 treatment did produce 92% female adults. Sexually dimorphic fin traits were quantified to look for potential effects of triclosan and E2 on the development of secondary sexual Characters. These results do not support the hypothesis that triclosan is potently estrogenic. However, changes in fin length and non-significant trends in sex ratio suggest triclosan is potentially weakly androgenic. (C) 2000 Elsevier Science Ltd. All rights reserved.