Lack of interaction of rhodopsin chromophore with membrane lipids. An electron-electron double resonance study using 14N:15N pairs.
Lack of interaction of rhodopsin chromophore with membrane lipids. An electron-electron double resonance study using 14N:15N pairs.
复制标题
视紫红质发色团与膜脂缺乏相互作用。
DOI：
10.1016/s0006-3495(88)83113-8
复制
发表时间：
1988
影响因子：
3.4
通讯作者：
Feix,JB
中科院分区：
文献类型：
--
作者：
Renk,GE;Crouch,RK;Feix,JB
电子-电子双共振（ELDOR）已被应用于研究特定的相互作用的15 N-自旋标记的硬脂酸与视紫红质类似物的视网膜生色团含有14 N自旋标记的视网膜。5和16自旋标记的硬脂酸被纳入含有自旋标记的色素的杆外段膜的脂质双层中。没有15 N和14 N自旋标记之间的相互作用，观察视紫红质或metarhodopsin II状态与这些标记的硬脂酸。因此，在该系统中，发色团的环部分必须与视紫红质和变视紫红质II形式的磷脂双层高度隔离。
Electron-electron double resonance (ELDOR) has been applied to the study of specific interactions of 15N-spin-labeled stearic acid with the retinal chromophore of a rhodopsin analogue containing a 14N spin-labeled retinal. Both the 5 and 16 spin-labeled stearic acids were incorporated into the lipid bilayer of rod outer segment membranes containing the spin-labeled pigment. No interaction between the 15N and 14N spin-labels was observed in rhodopsin or the metarhodopsin II state with either of these labeled stearic acids. Therefore in this system the ring portion of the chromophore must be highly sequestered from the phospholipid bilayer in both the rhodopsin and metarhodopsin II forms.