Biostable L-DNAzyme for Sensing of Metal Ions in Biological Systems.
Biostable L-DNAzyme for Sensing of Metal Ions in Biological Systems.
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用于传感生物系统中金属离子的生物稳定 L-DNAzyme
DOI：
10.1021/acs.analchem.5b04170
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发表时间：
2016-02-02
影响因子：
7.4
通讯作者：
Tan W
中科院分区：
文献类型：
--
作者：
Cui L;Peng R;Fu T;Zhang X;Wu C;Chen H;Liang H;Yang CJ;Tan W
DNA酶是一类重要的金属离子依赖性功能核酸，广泛应用于生物分析和生物医学领域。然而，DNA酶在实际应用中的使用受到天然核酸固有缺陷的阻碍，例如来自核酸酶消化和蛋白质结合的干扰，以及与其他核酸的不期望的分子间相互作用。基于D-DNAzyme的对映体L-DNAzyme具有互补的手性底物特异性，它可以与同一个非手性金属离子作为辅因子启动催化裂解活性。同时，利用非生物L-DNAzyme不受生物基质干扰的优点作为识别单元，构建了一种简单、稳定的L-DNAzyme传感器，用于检测复杂生物样品和活细胞中的金属离子。
DNAzymes, an important type of metal ion-dependent functional nucleic acid, are widely applied in bioanalysis and biomedicine. However, the use of DNAzymes in practical applications has been impeded by the intrinsic drawbacks of natural nucleic acids, such as interferences from nuclease digestion and protein binding, as well as undesired intermolecular interactions with other nucleic acids. On the basis of reciprocal chiral substrate specificity, the enantiomer of D-DNAzyme, L-DNAzyme, could initiate catalytic cleavage activity with the same achiral metal ion as a cofactor. Meanwhile, by using the advantage of nonbiological L-DNAzyme, which is not subject to the interferences of biological matrixes, as recognition units, a facile and stable L-DNAzyme sensor was proposed for sensing metal ions in complex biological samples and live cells.