The migration of mitochondrial DNA fragments to the nucleus affects the chronological aging process of Saccharomyces cerevisiae
The migration of mitochondrial DNA fragments to the nucleus affects the chronological aging process of Saccharomyces cerevisiae
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DOI：
10.1111/j.1474-9726.2010.00607.x
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发表时间：
2010-10-01
期刊：
影响因子：
7.8
通讯作者：
Ivessa, Andreas S.
中科院分区：
文献类型：
--
作者：
Cheng, Xin;Ivessa, Andreas S.
片段化的线粒体DNA（mtDNA）迁移到细胞核已被证明发生在包括酵母，植物和哺乳动物在内的多个物种中。几种人类疾病，包括Pallister-Hall综合征和粘脂沉积症，可以通过mtDNA插入核DNA突变引发。在酵母中，我们证明了线粒体DNA片段易位到细胞核的速率在时间老化过程中增加。酵母的时间寿命（CLS）是由非分裂细胞群的存活决定的。而线粒体DNA片段的迁移率升高的酵母菌株的细胞核表现出加速的时间老化，线粒体DNA转移率降低的菌株的细胞核表现出延长CLS。虽然最流行的衰老理论之一是自由基理论，但线粒体DNA片段迁移到细胞核也可能通过增加高龄细胞中核基因组的不稳定性而促进时序衰老过程。
P>Migration of fragmented mitochondrial DNA (mtDNA) to the nucleus has been shown to occur in multiple species including yeast, plants, and mammals. Several human diseases, including Pallister-Hall syndrome and mucolipidosis, can be initiated by mtDNA insertion mutagenesis of nuclear DNA. In yeast, we demonstrated that the rate of mtDNA fragments translocating to the nucleus increases during chronological aging. The yeast chronological lifespan (CLS) is determined by the survival of nondividing cell populations. Whereas yeast strains with elevated migration rates of mtDNA fragments to the nucleus showed accelerated chronological aging, strains with decreased mtDNA transfer rates to the nucleus exhibited an extended CLS. Although one of the most popular theories of aging is the free radical theory, migration of mtDNA fragments to the nucleus may also contribute to the chronological aging process by possibly increasing nuclear genomic instability in cells with advanced age.