Comprehensive Analysis of Interaction Networks of Telomerase Reverse Transcriptase with Multiple Bioinformatic Approaches: Deep Mining the Potential Functions of Telomere and Telomerase
Comprehensive Analysis of Interaction Networks of Telomerase Reverse Transcriptase with Multiple Bioinformatic Approaches: Deep Mining the Potential Functions of Telomere and Telomerase
复制标题
多种生物信息学手段综合分析端粒酶反转录酶相互作用网络：深度挖掘端粒和端粒酶的潜在功能
DOI：
10.1089/rej.2016.1909
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发表时间：
2017-08-01
影响因子：
2.6
通讯作者：
Geng, Xin
中科院分区：
文献类型：
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作者：
Hou, Chunyu;Wang, Fei;Geng, Xin
端粒酶逆转录酶（TERT）是端粒酶复合物的蛋白质成分。有证据表明，TERT的非脑函数与端粒伸长无关。但是，尚未明确揭示有关TERT与其靶基因之间相互作用的机制。 TERT的生物学功能尚未完全阐明，到目前为止被低估了。为了进一步探索这些功能，我们使用多个生物信息学数据基础研究了TERT相互作用网络，包括Biogrid，String，David，David，Genecards，Genemania，Genemania，Panther，Mirwalk，Mirtarbase，Mirtarbase，Mirnet，Mirnet，MirdB和TargetScan。此外，使用Cytoscape软件构建了网络图。由于竞争性内源性RNA（CERNAS）是内源性转录本，通过使用共享miRNA识别元素竞争MicroRNA（miRNA）的结合，因此它们参与创建广泛的调节网络。因此，在这项研究中还研究了TERT的CERNA监管网络。有趣的是，我们发现TERT相互作用网络和CERNAS靶基因都存在三个基因PABPC1，SLC7A11和TP53。据预测，TERT可能在某些罕见疾病的产生或发展中扮演非脑作用，例如裂谷热和障碍症。因此，我们的数据将有助于破译TERT的相互作用网络，并揭示端粒酶在癌症和衰老相关疾病中的未知功能。
Telomerase reverse transcriptase (TERT) is the protein component of telomerase complex. Evidence has accumulated showing that the nontelomeric functions of TERT are independent of telomere elongation. However, the mechanisms governing the interaction between TERT and its target genes are not clearly revealed. The biological functions of TERT are not fully elucidated and have thus far been underestimated. To further explore these functions, we investigated TERT interaction networks using multiple bioinformatic data-bases, including BioGRID, STRING, DAVID, GeneCards, GeneMANIA, PANTHER, miRWalk, mirTarBase, miRNet, miRDB, and TargetScan. In addition, network diagrams were built using Cytoscape software. As competing endogenous RNAs (ceRNAs) are endogenous transcripts that compete for the binding of microRNAs (miRNAs) by using shared miRNA recognition elements, they are involved in creating widespread regulatory networks. Therefore, the ceRNA regulatory networks of TERT were also investigated in this study. Interestingly, we found that the three genes PABPC1, SLC7A11, and TP53 were present in both TERT interaction networks and ceRNAs target genes. It was predicted that TERT might play nontelomeric roles in the generation or development of some rare diseases, such as Rift Valley fever and dyscalculia. Thus, our data will help to decipher the interaction networks of TERT and reveal the unknown functions of telomerase in cancer and aging-related diseases.