Impacts of soil salinity on Bt protein concentration in square of transgenic Bt cotton.
Impacts of soil salinity on Bt protein concentration in square of transgenic Bt cotton.
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土壤盐分对转基因Bt棉方格Bt蛋白浓度的影响
DOI：
10.1371/journal.pone.0207013
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Chen DH
中科院分区：
文献类型：
--
作者：
Wang YH;Gao J;Sun MF;Chen JP;Zhang X;Chen Y;Chen DH
转Bt基因抗虫棉的抗虫性因植株器官和环境条件的不同而不同。本研究旨在探讨土壤盐分对棉花Bt蛋白含量的影响，并阐明Bt药效降低的可能机制。2015年和2016年，两个棉花品种（NuCOTN 33 B和CCRI 07，盐敏感和耐盐）在田间条件下在4个自然盐分水平下进行了盐胁迫，2017年在温室条件下进行了7个土壤盐分水平，范围为0.5至18.8 dS m-1。田间试验结果表明，在盐度为7.13 dS m-1时，Bt蛋白含量变化不显著，而在盐度为10.41和14.16 dS m-1时，Bt蛋白含量显著下降。温室试验进一步表明，当土壤盐分超过9.1 dS m-1时，检测到方形杀虫蛋白含量的显着下降趋势。同时，高盐胁迫显著降低了棉蕾可溶性蛋白质和全氮含量，降低了硝酸还原酶（NR）、谷氨酰胺合成酶（GS）和谷氨酰胺转氨酶（GPT）活性，提高了氨基酸含量、蛋白酶和肽酶活性。高盐胁迫还降低了根活力（RV）、根总吸收面积（RTA）和根活跃吸收面积（RAA）。NuCOTN 33 B的Bt蛋白含量下降幅度大于CCRI 07，而CCRI 07具有较强的方形蛋白合成酶活性和较高的RV、RTA和RAA水平。因此，本研究结果表明，高盐胁迫（≥ 9.1dS m-1）显著影响棉蕾杀虫蛋白的表达，棉蕾蛋白质合成减少、蛋白质降解增加和根代谢活性降低可能导致棉蕾Bt蛋白含量下降。
Insect-resistance of transgenic Bacillus thuringiensis (Bt) cotton varies among plants organs and with different environmental conditions. The objective of this study was to examine the influence of soil salinity on Bt protein concentration in cotton squares and to elucidate the potential mechanism of Bt efficacy reduction. Two cotton cultivars (NuCOTN 33B and CCRI 07, salt-sensitive and salt-tolerant) were subjected to salinity stress under four natural saline levels in field conditions in 2015 and 2016 and seven regimes of soil salinity ranged from 0.5 to 18.8 dS m-1 in greenhouse conditions in 2017. Results of field studies revealed that Bt protein content was not significantly changed at 7.13 dS m-1 salinity, but exhibited a significant drop at the 10.41 and 14.16 dS m-1 salinity. The greenhouse experiments further showed similar trends that significant declines of the insecticidal protein contents in squares were detected when soil salinity exceeded 9.1 dS m-1. Meanwhile, high salinity resulted in significant reduction in contents of soluble protein and total nitrogen, activities of nitrate reductase (NR), glutamine synthetase (GS) and glutamic-pyruvic transaminase (GPT), but increased amino acid content, activities of protease and peptidase in cotton squares. High salinity also decreased root vigor (RV), root total absorption area (RTA) and root active absorption area (RAA). The extent of decrease of Bt protein content was more pronounced in NuCOTN 33B than CCRI 07, and CCRI07 exhibited stronger enzymes activities involved in square protein synthesis and higher levels of RV, RTA and RAA. Therefore, the results of our present study indicated that insecticidal protein expression in cotton squares were significantly affected by higher salinity (equal to or higher than 9.1 dS m-1), reduced protein synthesis and increased protein degradation in squares and reduced metabolic activities in roots might lead to the decrease of Bt protein content in squares.
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影响因子：
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作者：
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作者：
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影响因子：
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作者：
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影响因子：
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作者：
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DOI：
10.2478/v10146-011-0011-x
发表时间：
2011-01-01
期刊：
Journal of Agrobiology
影响因子：
--
作者：
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通讯作者：
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