Nanostructured surfaces for analysis of anticancer drug and cell diagnosis based on electrochemical and SERS tools.
Nanostructured surfaces for analysis of anticancer drug and cell diagnosis based on electrochemical and SERS tools.
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DOI：
10.1186/s40580-018-0143-4
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发表时间：
2018
期刊：
影响因子：
11.7
通讯作者：
Choi JW
中科院分区：
文献类型：
--
作者：
El-Said WA;Yoon J;Choi JW
发现新的抗癌药物并筛选其疗效需要大量的资源和耗时的过程。为了减少发现新的抗癌药物所需的时间和资源，已经发展了快速、灵敏和无损的体外和体内检测抗癌药物的效果和作用机制的方法。为了在体外和体内检测抗癌药物的有效性、分布和作用机制，基于纳米结构的表面，发展了电化学芯片等电化学技术和表面增强拉曼光谱(SERS)等光学技术的应用。本文综述了电化学细胞芯片和表面增强拉曼散射技术的研究进展，基于纳米结构表面的电化学细胞芯片已被开发用于体外细胞活性检测和抗癌药物效果的评价，显示出在低浓度下评价几种化学物质的细胞毒作用的高能力。基于纳米结构表面的表面增强拉曼光谱技术是一种无标记、简单、无损的技术，可用于在细胞水平上监测不同抗癌药物的分布、作用机制和代谢。利用电化学芯片和基于纳米结构表面的表面增强拉曼光谱技术可以很好地检测抗癌药物的作用和作用机制。
Discovering new anticancer drugs and screening their efficacy requires a huge amount of resources and time-consuming processes. The development of fast, sensitive, and nondestructive methods for the in vitro and in vivo detection of anticancer drugs’ effects and action mechanisms have been done to reduce the time and resources required to discover new anticancer drugs. For the in vitro and in vivo detection of the efficiency, distribution, and action mechanism of anticancer drugs, the applications of electrochemical techniques such as electrochemical cell chips and optical techniques such as surface-enhanced Raman spectroscopy (SERS) have been developed based on the nanostructured surface. Research focused on electrochemical cell chips and the SERS technique have been reviewed here; electrochemical cell chips based on nanostructured surfaces have been developed for the in vitro detection of cell viability and the evaluation of the effects of anticancer drugs, which showed the high capability to evaluate the cytotoxic effects of several chemicals at low concentrations. SERS technique based on the nanostructured surface have been used as label-free, simple, and nondestructive techniques for the in vitro and in vivo monitoring of the distribution, mechanism, and metabolism of different anticancer drugs at the cellular level. The use of electrochemical cell chips and the SERS technique based on the nanostructured surface should be good tools to detect the effects and action mechanisms of anticancer drugs.