A negative loop within the nuclear pore complex controls global chromatin organization.
A negative loop within the nuclear pore complex controls global chromatin organization.
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DOI：
10.1101/gad.264341.115
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发表时间：
2015-09-01
影响因子：
10.5
通讯作者：
Ohkura H
中科院分区：
文献类型：
--
作者：
Breuer M;Ohkura H
Breuer和Ohkura提出了核孔复合物（NPC）内控制染色质附着状态的负调控环，其中Nup155和Nup93招募Nup62以抑制Nup155对染色质的束缚。核孔复合物（NPC）系留染色质以创造基因调控的环境，但关于如何调节这种活性以避免基因组过度系留的知之甚少。在这里，我们提出了一个负调控环内的NPC控制染色质附着状态，其中Nup155和Nup93招募Nup62抑制染色质束缚Nup155。Nup62的缺失严重破坏了雌性生殖系和体细胞核中的染色质分布，这可以通过编码Nup155来逆转。因此，NPC中的这种通用调节系统对于控制细胞核中的大规模染色质组织至关重要。
Breuer and Ohkura propose a negative regulatory loop within the nuclear pore complex (NPC) controlling the chromatin attachment state, in which Nup155 and Nup93 recruit Nup62 to suppress chromatin tethering by Nup155. The nuclear pore complex (NPC) tethers chromatin to create an environment for gene regulation, but little is known about how this activity is regulated to avoid excessive tethering of the genome. Here we propose a negative regulatory loop within the NPC controlling the chromatin attachment state, in which Nup155 and Nup93 recruit Nup62 to suppress chromatin tethering by Nup155. Depletion of Nup62 severely disrupts chromatin distribution in the nuclei of female germlines and somatic cells, which can be reversed by codepleting Nup155. Thus, this universal regulatory system within the NPC is crucial to control large-scale chromatin organization in the nucleus.