Leukocyte and complement activation by GM1-specific antibodies is associated with acute motor axonal neuropathy in rabbits
Leukocyte and complement activation by GM1-specific antibodies is associated with acute motor axonal neuropathy in rabbits
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DOI：
10.1016/j.jneuroim.2006.10.005
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发表时间：
2007-01-01
影响因子：
3.3
通讯作者：
van der Pol, W-Ludo
中科院分区：
文献类型：
--
作者：
van Sorge, Nina M.;Yuki, Nobuhiro;van der Pol, W-Ludo
人类急性运动轴索神经病（AMAN）与GM1特异性抗体的存在有关。用含有GM1的神经节苷脂混合物、纯化的GM1或展示GM1样结构的空肠弯曲杆菌脂寡糖免疫兔子可产生GM1特异性抗体，但轴突性多神经病仅发生在一个动物亚组中。本研究的目的是解剖的分子基础的可变诱导AMAN在兔。因此，我们分析了神经节苷脂免疫家兔血浆样本中GM1特异性抗体的促炎特征，有和没有神经功能缺损。来自所有患有AMAN的兔的GM1特异性血浆样品能够激活补体和白细胞，而来自没有麻痹的兔的血浆样品则没有。此外，GM1特异性IgG介导的白细胞活化在临床症状出现之前就已检测到。这些数据表明，AMAN仅发生在产生具有促炎特性的GM1特异性抗体的兔子中。(c)2006 Elsevier B.V.保留所有权利。
Acute motor axonal neuropathy (AMAN) in humans is associated with the presence of GM1-specific antibodies. Immunization of rabbits with GM1-containing ganglioside mixtures, purified GM1, or Campylobacter jejuni lipo-oligosaccharide exhibiting a GM1-like structure elicits GM1-specific antibodies, but axonal polyncuropathy only occurs in a subset of animals. This study aimed to dissect the molecular basis for the variable induction of AMAN in rabbits. Therefore, we analyzed the proinflammatory characteristics of GM1-specific antibodies in plasma samples from ganglioside-immunized rabbits with and without neurological deficits. GM1-specific plasma samples from all rabbits with AMAN were capable of activating both complement and leukocytes, in contrast to none of the plasma samples from rabbits without paralysis. Furthermore, GM1-specific 1gG-mediated activation of leukocytes was detected before the onset of clinical signs. These data suggest that AMAN only occurs in rabbits that develop GM1-specific antibodies with pro-inflammatory properties. (c) 2006 Elsevier B.V. All rights reserved.