Ultrasound assessment of inflammation and renal tissue injury with microbubbles targeted to P-selectin
Ultrasound assessment of inflammation and renal tissue injury with microbubbles targeted to P-selectin
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DOI：
10.1161/hc4201.097061
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发表时间：
2001-10-23
期刊：
影响因子：
37.8
通讯作者：
Ley, K
中科院分区：
文献类型：
--
作者：
Lindner, JR;Song, J;Ley, K
背景：目前还没有能够无创评估组织炎症的常规方法。我们假设，针对内皮细胞粘附分子p -选择素的组织微泡保留将为超声成像评估炎症提供一种手段。方法与结果：将抗p -选择素单克隆抗体偶联到脂质壳上，制备了靶向p -选择素（MBp）的磷脂微泡。采用活体显微镜观察小鼠和肿瘤坏死因子(TNF)- α刺激的野生型小鼠，观察无抗体（MB）和有同型对照抗体（MBiso）的MBp和对照微泡的微血管行为。所有微泡的保留率均增加(P
Background-Routine methods capable of assessing tissue inflammation noninvasively are currently not available. We hypothesized that tissue retention of microbubbles targeted to the endothelial cell adhesion molecule P-selectin would provide a means to assess inflammation with ultrasound imaging.Methods and Results-Phospholipid microbubbles targeted to P-selectin (MBp) were created by conjugating monoclonal antibodies against murine P-selectin to the lipid shell. The microvascular behaviors of MBp and control microbubbles without antibody (MB) or with isotype control antibody (MBiso) were assessed by intravital microscopy of cremasteric venules of control and tumor necrosis factor (TNF)-alpha -stimulated wild-type mice. Retention of all microbubbles increased (P