Analytical Method for Experimental Validation of Computer-Designed Antibody
Analytical Method for Experimental Validation of Computer-Designed Antibody
复制标题
计算机设计抗体实验验证的分析方法
DOI：
10.1007/978-1-0716-2609-2_23
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发表时间：
2022
影响因子：
--
通讯作者：
Tsumoto Kouhei
中科院分区：
文献类型：
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作者：
Tanabe Aki;Tsumoto Kouhei
在抗体的计算设计中，目标抗原和抗体之间的相互作用分析是获取反馈以验证和优化设计的重要过程。动力学和热力学参数以及结合亲和力 (KD) 允许对分子识别进行更详细的评估和理解。在本章中，我们总结了计算KD值（ELISA、FP）、分析与实际细胞的结合活性（FCM）以及评估动力学和热力学参数（ITC、SPR、BLI）的常规实验方法，包括高通量分析和最近开发的实验技术。
In the computational design of antibodies, the interaction analysis between target antigen and antibody is an essential process to obtain feedback for validation and optimization of the design. Kinetic and thermodynamic parameters as well as binding affinity (KD) allow for a more detailed evaluation and understanding of the molecular recognition. In this chapter, we summarize the conventional experimental methods which can calculateKDvalue (ELISA, FP), analyze a binding activity to actual cells (FCM), and evaluate the kinetic and thermodynamic parameters (ITC, SPR, BLI), including high-throughput analysis and a recently developed experimental technique.