Heterogeneity in the Infection Biology of Campylobacter jejuni Isolates in Three Infection Models Reveals an Invasive and Virulent Phenotype in a ST21 Isolate from Poultry.
Heterogeneity in the Infection Biology of Campylobacter jejuni Isolates in Three Infection Models Reveals an Invasive and Virulent Phenotype in a ST21 Isolate from Poultry.
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DOI：
10.1371/journal.pone.0141182
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Wigley P
中科院分区：
文献类型：
--
作者：
Humphrey S;Lacharme-Lora L;Chaloner G;Gibbs K;Humphrey T;Williams N;Wigley P
虽然弯曲杆菌是世界上细菌性食源性胃肠炎的主要原因，家禽作为感染源的重要性也得到了很好的理解，但对其在肉鸡中的感染生物学知之甚少。我们对空肠弯曲菌生物学的了解大部分是基于大多数实验室研究中使用的少量分离株感染近交系或SPF实验室鸡系。近年来，我们的研究表明，无论是宿主反应还是微生物生态学，C。肉鸡空肠内的C.空肠分离株。在这里，我们描述的异质性感染内的一组C。空肠分离株在两个肉鸡品种、人肠道上皮细胞和Galleria昆虫模型中的毒力。全是C。空肠分离物定殖于鸡盲肠，尽管不同分离物之间胃肠道其它部分的定殖不同。肠外传播至肝脏的情况在分离株和禽类品种之间存在差异，但家禽分离株13126（序列类型21）在两种肉鸡品种中显示出最高水平的肠外传播至肝脏，超过70%的快速生长品种的禽类和50%的缓慢生长品种的禽类具有C.肝脏中的空肠重要的是，13126在人肠上皮细胞中的侵袭性显著高于其他分离株，并且在Galleria感染模型中的死亡率最高。总之，我们的研究结果表明，不仅有相当大的异质性感染生物学的C。在禽类、哺乳动物和替代模型中，空肠的分离株具有侵袭性和毒性表型。具有侵袭性表型的分离株将对鸡生产造成重大风险，增加控制难度，加上13126中观察到的毒力表型，可能会增加对公共卫生的风险。
Although Campylobacter is the leading cause of bacterial foodborne gastroenteritis in the world and the importance of poultry as a source of infection is well understood we know relatively little about its infection biology in the broiler chicken. Much of what we know about the biology of Campylobacter jejuni is based on infection of inbred or SPF laboratory lines of chickens with a small number of isolates used in most laboratory studies. Recently we have shown that both the host response and microbial ecology of C. jejuni in the broiler chicken varies with both the host-type and significantly between C. jejuni isolates. Here we describe heterogeneity in infection within a panel of C. jejuni isolates in two broiler chicken breeds, human intestinal epithelial cells and the Galleria insect model of virulence. All C. jejuni isolates colonised the chicken caeca, though colonisation of other parts of the gastrointestinal tract varied between isolates. Extra-intestinal spread to the liver varied between isolates and bird breed but a poultry isolate 13126 (sequence type 21) showed the greatest levels of extra-intestinal spread to the liver in both broiler breeds with over 70% of birds of the fast growing breed and 50% of the slower growing breed having C. jejuni in their livers. Crucially 13126 is significantly more invasive than other isolates in human intestinal epithelial cells and gave the highest mortality in the Galleria infection model. Taken together our findings suggest that not only is there considerable heterogeneity in the infection biology of C. jejuni in avian, mammalian and alternative models, but that some isolates have an invasive and virulent phenotype. Isolates with an invasive phenotype would pose a significant risk and increased difficulty in control in chicken production and coupled with the virulent phenotype seen in 13126 could be an increased risk to public health.