Photo-control of the mitotic kinesin Eg5 using a novel photochromic inhibitor composed of a spiropyran derivative
Photo-control of the mitotic kinesin Eg5 using a novel photochromic inhibitor composed of a spiropyran derivative
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使用由螺吡喃衍生物组成的新型光致变色抑制剂对有丝分裂驱动蛋白 Eg5 进行光控制
DOI：
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
S. Maruta
中科院分区：
文献类型：
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作者：
Kei Sadakane;M. Takaichi;S. Maruta
本研究中，我们合成了一种新型的有丝分裂驱动蛋白Eg 5的光致变色抑制剂，它是由光致变色化合物螺吡喃组成的，以光控制Eg 5的功能。化合物（S）-2，3-二螺吡喃丙酸（DSPPA）在可见光或紫外光照射下表现出可逆的螺吡喃-汞光异构化。DSPPA对Eg 5 ATP酶和运动活动的抑制作用引起可逆性变化，这与螺吡喃-汞的光异构化有关。微管依赖性ATP酶活性显着抑制螺吡喃异构体的DSPPA比mercury异构体。此外，在体外运动试验表明，微管滑动速度减少螺吡喃异构体比mercidazole异构体。这表明螺吡喃衍生物可用于调节有丝分裂驱动蛋白的功能。
In this study, we synthesized a novel photochromic inhibitor of the mitotic kinesin Eg5, which is composed of the photochromic compound spiropyran to photo-control the function of Eg5. The compound (S)-2, 3-dispiropyran propionic acid (DSPPA) exhibits reversible spiropyran-merocyanine photo-isomerization upon irradiation with visible or ultra-violet light. DSPPA induced reversible changes in the inhibitory effect on Eg5 ATPase and motor activities, which correlates with the spiropyran-merocyanine photo-isomerization. Microtubule-dependent ATPase activity was significantly more inhibited by the spiropyran isomer of DSPPA than by the merocyanine isomer. Additionally, an in vitro motility assay revealed that the microtubule gliding velocity was reduced more by the spiropyran isomer than by the merocyanine isomer. This indicates that the spiropyran derivative may be useful in regulating the function of the mitotic kinesin.
DOI：
10.1073/pnas.85.17.6314
发表时间：
1988-09-01
影响因子：
11.1
作者：
HACKNEY, DD
通讯作者：
HACKNEY, DD
影响因子：
56.9
作者：
Mayer, TU;Kapoor, TM;Mitchison, TJ
通讯作者：
Mitchison, TJ