REACTION BETWEEN ELASTASE AND ELASTIC TISSUE .4. SOLUBLE ELASTINS
REACTION BETWEEN ELASTASE AND ELASTIC TISSUE .4. SOLUBLE ELASTINS
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DOI：
10.1042/bj0800121
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发表时间：
1961-01-01
影响因子：
4.1
通讯作者：
CZERKAWSKI, JW
中科院分区：
文献类型：
--
作者：
HALL, DA;CZERKAWSKI, JW
本文介绍了几种制备可溶性弹性蛋白的方法，结果表明，通过控制弹性蛋白的水解可获得最好的材料。凝聚被认为是[α]-弹性蛋白的最佳表征，并研究了测量这种现象的最合适条件。本文还研究了洗涤剂和弹性蛋白酶对这一现象的影响。结果表明，α-弹性蛋白的凝聚过程是复杂的，主要是由蛋白质分子的带电中心之间的盐样相互作用引起的。[α]-弹性蛋白和弹性蛋白酶之间反应的动力学研究给出了一个经验方程。它表明，只有一些合理的假设和观察到的事实导致一个理论方程是相同的形式作为经验之一。该方程的常数具有良好的物理意义，可以通过实验获得。
Several methods of preparation of soluble elastin are described and it is shown that the best material is obtained by controlled elastolysis. Coacervation is taken as best characterization of [alpha]-elastin and the most suitable conditions for the measurement of this phenomenon are investigated. The effect of detergents and elastase on this phenomenon are also studied. It is concluded that the process of coacervation of [alpha]-elastin is complex and is mainly brought about by salt-like interaction between Charged centers of the protein molecules. The kinetic studies of the reaction between [alpha]-elastin and elastase give rise to an empirical equation. It is shown that only a few reasonable assumptions and the observed facts lead to a theoretical equation which is of the same form as the empirical one. The constants of the equation have a sound physical meaning and can be obtained experimentally.