DNA barcoding insect-host plant associations
DNA barcoding insect-host plant associations
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DOI：
10.1098/rspb.2008.1264
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发表时间：
2009-02-22
影响因子：
4.7
通讯作者：
Gomez-Zurita, Jesus
中科院分区：
文献类型：
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作者：
Jurado-Rivera, Jose A.;Vogler, Alfried P.;Gomez-Zurita, Jesus
广泛用于植物鉴定和编目的短序列片段（“DNA条形码”）仍然适用于复杂的生物学问题。宿主-草食动物相互作用是地球上大部分物种共同进化关系的基础，但它们的研究经常受到有限或不可靠的宿主记录的阻碍。在这里，我们证明了DNA条形码可以大大改善这种情况，因为它们（i）提供了一个安全的识别宿主植物物种和（ii）建立营养协会的真实性。利用叶绿体trnL（UAA）内含子作为条形码，从甲虫DNA提取物中扩增，鉴定来自澳大利亚的叶甲（亚科叶甲亚科）的寄主植物。序列相似性和系统发育分析提供了精确的识别每个主机物种在部落，属和具体的水平，这取决于现有的数据库覆盖范围在各种植物谱系。包括76种-超过10%的已知澳大利亚动物群-饲料的13个植物科，与澳大利亚辐射的桃金娘科（桉树）和豆科（阿卡西亚斯）的偏好。甲虫的系统发育分析表明，一般的主机协会的保护，但与罕见的主机之间的遥远的植物谱系的变化，包括一些情况下，条形码支持两个同源性遥远的宿主植物。这项研究表明，植物条形码已经是可行的，目前公开的数据。通过直接从植食性甲虫的DNA提取物中测序植物条形码，为宿主协会提供了强有力的物理证据。因此，使用短DNA片段的分子鉴定将物种的检测和它们的相互作用的分析结合在一起。
Short-sequence fragments ('DNA barcodes') used widely for plant identification and inventorying remain to be applied to complex biological problems. Host-herbivore interactions are fundamental to coevolutionary relationships of a large proportion of species on the Earth, but their study is frequently hampered by limited or unreliable host records. Here we demonstrate that DNA barcodes can greatly improve this situation as they (i) provide a secure identification of host plant species and (ii) establish the authenticity of the trophic association. Host plants of leaf beetles (subfamily Chrysomelinae) from Australia were identified using the chloroplast trnL(UAA) intron as barcode amplified from beetle DNA extracts. Sequence similarity and phylogenetic analyses provided precise identifications of each host species at tribal, generic and specific levels, depending on the available database coverage in various plant lineages. The 76 species of Chrysomelinae included-more than 10 per cent of the known Australian fauna-feed on 13 plant families, with preference for Australian radiations of Myrtaceae (eucalypts) and Fabaceae (acacias). Phylogenetic analysis of beetles shows general conservation of host association but with rare host shifts between distant plant lineages, including a few cases where barcodes supported two phylogenetically distant host plants. The study demonstrates that plant barcoding is already feasible with the current publicly available data. By sequencing plant barcodes directly from DNA extractions made from herbivorous beetles, strong physical evidence for the host association is provided. Thus, molecular identification using short DNA fragments brings together the detection of species and the analysis of their interactions.