Determining the Replication Kinetics and Cellular Tropism of Influenza D Virus on Primary Well-Differentiated Human Airway Epithelial Cells
Determining the Replication Kinetics and Cellular Tropism of Influenza D Virus on Primary Well-Differentiated Human Airway Epithelial Cells
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DOI：
10.3390/v11040377
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发表时间：
2019-04-01
期刊：
影响因子：
4.7
通讯作者：
Dijkman, Ronald
中科院分区：
文献类型：
--
作者：
Holwerda, Melle;Kelly, Jenna;Dijkman, Ronald
流感病毒是臭名昭著的病原体，经常跨越物种屏障，对动物和人类健康造成严重后果。 2011 年，在猪的呼吸道中发现了正粘病毒科的一种新成员 D 型流感病毒 (IDV)。流行病学调查显示，IDV 在世界范围内的牲畜中传播，并且在职业接触牲畜的人类中检测到了针对 IDV 的抗体。为了确定 IDV 向人类的传播能力，我们使用人类体外呼吸道上皮模型确定了病毒复制动力学和细胞向性。 IDV 的接种揭示了在不同体温下有效的复制动力学和顶端后代病毒的释放。有趣的是，尽管 IDV 具有对纤毛细胞的细胞趋向性偏好，但与丙型流感病毒相比，IDV 的复制特征显示出更高的复制动力学。总的来说，这些结果可能表明为什么在职业接触牲畜的人类中检测到 IDV 定向抗体。
Influenza viruses are notorious pathogens that frequently cross the species barrier with often severe consequences for both animal and human health. In 2011, a novel member of the Orthomyxoviridae family, Influenza D virus (IDV), was identified in the respiratory tract of swine. Epidemiological surveys revealed that IDV is distributed worldwide among livestock and that IDV-directed antibodies are detected in humans with occupational exposure to livestock. To identify the transmission capability of IDV to humans, we determined the viral replication kinetics and cell tropism using an in vitro respiratory epithelium model of humans. The inoculation of IDV revealed efficient replication kinetics and apical progeny virus release at different body temperatures. Intriguingly, the replication characteristics of IDV revealed higher replication kinetics compared to Influenza C virus, despite sharing the cell tropism preference for ciliated cells. Collectively, these results might indicate why IDV-directed antibodies are detected among humans with occupational exposure to livestock.