Staphylococcus aureus plasmids without mobilization genes are mobilized by a novel conjugative plasmid from community isolates
Staphylococcus aureus plasmids without mobilization genes are mobilized by a novel conjugative plasmid from community isolates
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DOI：
10.1093/jac/dku454
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发表时间：
2015-03-01
影响因子：
5.2
通讯作者：
Grubb, W. B.
中科院分区：
文献类型：
--
作者：
O'Brien, F. G.;Ramsay, J. P.;Grubb, W. B.
目的：为了描述一个家庭的接合质粒从殖民社区金黄色葡萄球菌分离，并确定他们的能力，动员无关的抗菌素耐药性/毒力质粒，不编码动员functions.Methods：质粒pWBG 749标记Tn 551（pWBG 749 e），使实验室操作。将质粒pWBG 749 e与S.进行携带不相关质粒的七种不同谱系的金黄色葡萄球菌和动员实验。结果：pWBG 745、pWBG 748和pWBG 749与万古霉素耐药质粒pBRZ 01属于同一个接合质粒家族。质粒pWBG 749 e移动了5个不相关的质粒。动员质粒pWBG 744是一个pIB 485家族质粒，也存在于国际沙门氏菌中。结论：质粒pWBG 749 e可动员无关的金黄色葡萄球菌。金黄色葡萄球菌质粒，其传播方式以前还不清楚。
Objectives: To describe a family of conjugative plasmids isolated from colonizing community Staphylococcus aureus and determine their ability to mobilize unrelated antimicrobial resistance/virulence plasmids, not encoding mobilization functions.Methods: Plasmid pWBG749 was labelled with Tn551 (pWBG749e) to enable laboratory manipulation. Plasmid pWBG749e was conjugated into S. aureus of seven different lineages that harboured unrelated plasmids and mobilization experiments were performed. Plasmids were screened by EcoRI restriction and hybridization with probes prepared from unique pWBG749 conjugation genes.Results: Conjugative plasmids pWBG745, pWBG748 and pWBG749 belong to the same conjugative-plasmid family as the vancomycin resistance plasmid pBRZ01. Plasmid pWBG749e mobilized five unrelated plasmids. Mobilized plasmid pWBG744 is a pIB485-family plasmid that was also found in international S. aureus.Conclusions: Plasmid pWBG749e can mobilize unrelated S. aureus plasmids whose means of dissemination have not previously been understood.