Rapid determination of metformin in human plasma by liquid chromatography-tandem mass spectrometry method
Rapid determination of metformin in human plasma by liquid chromatography-tandem mass spectrometry method
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DOI：
10.1016/j.jchromb.2003.12.017
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发表时间：
2004-04-05
影响因子：
3
通讯作者：
Zhong, DF
中科院分区：
文献类型：
--
作者：
Chen, XY;Gu, Q;Zhong, DF
描述了一种快速、灵敏且特异的液相色谱-串联质谱方法，用于定量人血浆中的二甲双胍。使用乙腈进行简单的一步蛋白质沉淀后，二甲双胍和内标苯海拉明在 C-8 柱上进行色谱分析，并通过串联质谱法进行检测。选择大气压化学电离接口来减少样品基质成分的离子抑制并提供高灵敏度。该方法的色谱总运行时间为 3.4 分钟，并且在 2-2000 ng/ml 范围内呈线性。日内和日间精密度以相对标准偏差 (R.S.D.) 表示，范围分别为 4.4% 至 5.7% 和 1.3% 至 2.8%。就 %RE（相对误差）而言，测定准确度低于 1%。该测定用于评估 20 名健康志愿者口服含有 500 mg 二甲双胍和 2.5 mg 格列本脲的多组分制剂后二甲双胍的药代动力学。 (C) 2004 Elsevier B.V. 保留所有权利。
A rapid, sensitive and specific liquid chromatography-tandem mass spectrometry method is described for quantitation of metformin in human plasma. After a simple, one-step protein precipitation using acetonitrile, metformin and the internal standard diphenhydramine were chromatographed on a C-8 column and detected by tandem mass spectrometry. An atmospheric pressure chemical ionization interface was chosen to reduce ion suppression from sample matrix components and provide high sensitivity. The method has a chromatographic total run time of 3.4 min and was linear within the range 2-2000 ng/ml. Intra- and inter-day precision, expressed as the relative standard deviation (R.S.D.), ranged from 4.4 to 5.7% and from 1.3 to 2.8%, respectively. Assay accuracy was less than 1% in terms of %RE (relative error). The assay was used to evaluate the pharmacokinetics of metformin after an oral administration of multicomponent formulation containing 500 mg metformin and 2.5 mg glyburide to 20 healthy volunteers. (C) 2004 Elsevier B.V. All rights reserved.