FORM OF DNA AND NATURE OF INTERACTIONS WITH PROTEINS IN CHROMATIN
FORM OF DNA AND NATURE OF INTERACTIONS WITH PROTEINS IN CHROMATIN
复制标题
DOI：
10.1093/nar/5.3.835
复制
发表时间：
1978-01-01
影响因子：
14.9
通讯作者：
BRAHMS, J
中科院分区：
文献类型：
--
作者：
GOODWIN, DC;BRAHMS, J
对天然染色质和分离的核小体（来自小牛胸腺）的研究表明，DNA是B型或修饰的B型。这是由染色质、核小体（来自小牛胸腺）和DNA纤维的拉曼光谱测定的，并与X射线衍射研究直接相关。用X射线衍射和拉曼光谱对纤维中三种DNA（A、B和C）的拉曼光谱进行了表征。特别地，DNA的C型的拉曼光谱的特征在于约870 cm-1的带。首次用拉曼光谱研究了不同来源的染色质随非组蛋白含量的增加而变化。非组蛋白蛋白相互作用的位点似乎涉及鸟嘌呤的N7位置，而组蛋白核心在该位点不相互作用。提出染色质特异性识别的机制与大沟有关。
Studies of native chromatins and of isolated nucleosomes (from calf thymus) show that the DNA is in the B form or modified B form. This was determined by Raman spectroscopy of chromatins, of nucleosomes (from calf thymus) and of DNA fibres and directly correlated with X-ray diffraction studies. The Raman spectra of three forms of DNA (A, B and C) have been characterized in fibres both by X-ray diffraction and Raman spectroscopy on the same sample. In particular, the Raman spectrum of the C form of DNA is characterized by a band of about 870 cm−1. For the first time, chromatins of different origins with increasing content of non-histone proteins have been investigated by Raman spectroscopy. The site of interaction of the non-histone proteins appears to involve the N7 position of guanine while the histone core does not interact at this site. It is proposed that the mechanism of specific recognition in chromatin involves the large groove.