The concanavalin A model of acute hepatitis in mice
The concanavalin A model of acute hepatitis in mice
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DOI：
10.1177/0023677215572841
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发表时间：
2015-04-01
影响因子：
2.4
通讯作者：
Tacke, F.
中科院分区：
文献类型：
--
作者：
Heymann, F.;Hamesch, K.;Tacke, F.
植物凝集素刀豆蛋白A(ConA)静脉注射是一种广泛使用的小鼠急性免疫性肝炎模型。与其他几种急性肝损伤模型不同，ConA诱导的损伤主要是由T细胞的激活和募集到肝脏驱动的。因此，ConA模型在其发病机制方面具有独特的特征，并与人类免疫介导性肝炎(如自身免疫性肝炎、急性病毒性肝炎或导致免疫激活的不同药物毒性实体)具有重要的相似之处。然而，ConA模型具有相当大的可变性，这取决于化合物的制备、小鼠的遗传背景、性别、年龄和动物设施屏障的微生物环境。该标准操作程序(SOP)包括ConA应用的详细方案，包括ConA工作液的制备、动物的处理、适当条件和终点的选择以及有效的剂量发现。
The intravenous injection of the plant lectin concanavalin A (ConA) is a widely used model for acute immune-mediated hepatitis in mice. In contrast to several other models for acute hepatic damage, ConA-induced injury is primarily driven by the activation and recruitment of T cells to the liver. Hence, the ConA model has unique features with respect to its pathogenesis and important similarities to immune-mediated hepatitis in humans, such as autoimmune hepatitis, acute viral hepatitis or distinct entities of drug toxicity leading to immune activation. However, the ConA model has considerable variability, depending on the preparation of the compound, genetic background of the mice, sex, age and microbial environment of the animal facility barrier. This standard operating procedure (SOP) comprises a detailed protocol for the ConA application, including preparation of ConA working solution, handling of the animals, choice of the appropriate conditions and endpoints, as well as efficient dose-finding.