Feeding period restriction alters the expression of peripheral circadian rhythm genes without changing body weight in mice.
Feeding period restriction alters the expression of peripheral circadian rhythm genes without changing body weight in mice.
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进食期限制改变了外周三昼夜节律基因的表达，而无需改变小鼠体重。
DOI：
10.1371/journal.pone.0049993
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Kim JB
中科院分区：
文献类型：
--
作者：
Jang H;Lee G;Kong J;Choi G;Park YJ;Kim JB
越来越多的证据表明，生物钟与代谢调节密切相关。然而，生物钟受损是否是体重增加等代谢失调的直接原因尚不清楚。在这项研究中，我们证明将进食时间限制在白天或夜间并不会显着改变小鼠的体重增加。外周生物钟基因的表达因喂养时间限制而改变，而光调节下丘脑生物钟基因的表达不受正常食物（NCD）或高脂饮食（HFD）的影响。在肝脏中，生物钟基因（包括Bmal1、Clock和Per2）的表达模式因不同的喂养时间限制而改变。此外，肝脏中脂肪生成基因、糖异生基因和脂肪酸氧化相关基因的表达也因饲喂时间限制而改变。鉴于喂养时间限制不会影响 NCD 或 HFD 患者的体重增加，因此摄入的食物量可能是决定体重的关键因素。总的来说，这些数据表明喂养时间限制调节外周生物钟基因的表达，该基因与光敏感的下丘脑生物钟基因分离。
Accumulating evidence suggests that the circadian clock is closely associated with metabolic regulation. However, whether an impaired circadian clock is a direct cause of metabolic dysregulation such as body weight gain is not clearly understood. In this study, we demonstrate that body weight gain in mice is not significantly changed by restricting feeding period to daytime or nighttime. The expression of peripheral circadian clock genes was altered by feeding period restriction, while the expression of light-regulated hypothalamic circadian clock genes was unaffected by either a normal chow diet (NCD) or a high-fat diet (HFD). In the liver, the expression pattern of circadian clock genes, including Bmal1, Clock, and Per2, was changed by different feeding period restrictions. Moreover, the expression of lipogenic genes, gluconeogenic genes, and fatty acid oxidation-related genes in the liver was also altered by feeding period restriction. Given that feeding period restriction does not affect body weight gain with a NCD or HFD, it is likely that the amount of food consumed might be a crucial factor in determining body weight. Collectively, these data suggest that feeding period restriction modulates the expression of peripheral circadian clock genes, which is uncoupled from light-sensitive hypothalamic circadian clock genes.