Brassica rapa.
Brassica rapa.
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DOI：
10.1007/978-1-4939-1658-0_1
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发表时间：
2015
影响因子：
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通讯作者：
Tom Lawrenson;Cassandra Goldsack;Lars Ostergaard;Penny A C Hundleby née Sparrow
中科院分区：
文献类型：
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作者：
Tom Lawrenson;Cassandra Goldsack;Lars Ostergaard;Penny A C Hundleby née Sparrow
在本章中，我们概述了A。根癌农杆菌介导的转化方法。停顿4天龄的子叶外植体和基因型R-o-18。转化效率通常在1%的范围内实现（基于来自200个接种外植体的2个PCR阳性独立芽）。该系统已开发用于庆大霉素筛选。
Within this chapter we outline anA. tumefaciens-mediated transformation method forB. rapausing 4-day-old cotyledonary explants and the genotype R-o-18. Transformation efficiencies are typically achieved in the region of 1 % (based on 2 PCR-positive independent shoots from 200 inoculated explants). This system has been developed to work with gentamicin selection.