Germ tube induction in Candida albicans.
Germ tube induction in Candida albicans.
复制标题
白色念珠菌的生殖管诱导。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
P. Sullivan
中科院分区：
文献类型：
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作者：
M. Shepherd;C. Yin;S. P. Ram;P. Sullivan
本文描述了一种利用葡萄糖和谷氨酰胺作为底物从白色念珠菌酵母细胞中生产生殖管的可重复和简单的系统。在胚管形成过程中，干重增加了一倍，但细胞数量保持不变。虽然DNA含量在胚管形成的前4小时没有变化，但RNA含量增加了一倍以上。白色念珠菌胚孢子的DNA和RNA含量分别为4.5 × 10(-15) g /细胞和48 × 10(-15) g /细胞。制霉菌素、苯乙醇、2,4-二硝基苯酚、氮杂塞碱、水杨基羟肟酸和5-氟胞嘧啶都是有效的芽管形成抑制剂。半胱氨酸、氰化钾和多毒素D均不能阻止种子萌发。尿嘧啶和亮氨酸的结合在胚管形成过程中迅速发生。RNA合成抑制剂、放线菌素、虫草素和道诺霉素抑制种子萌发并抑制尿嘧啶掺入。翻译抑制剂木霉素、金苷三羧酸、嘌呤霉素和环己亚胺对胚芽管形成和亮氨酸结合均有抑制作用。
A reproducible and simple system for the production of germ tubes from yeast cells of Candida albicans using glucose and glutamine as substrates has been described. During germ tube formation there was a doubling of the dry weight but the number of cells remained constant. Although the DNA content did not change for the first 4 h of germ tube formation, the RNA content more than doubled. The DNA and RNA content of C. albicans blastospores are 4.5 x 10(-15) g per cell and 48 x 10(-15) g per cell respectively. Nystatin, phenethyl alcohol, 2,4-dinitrophenol, azaserine, salicylhydroxamic acid, and 5-fluorocytosine were all effective inhibitors of germ tube formation. Cysteine, potassium cyanide, and polyoxin D did not prevent germination. The incorporation of both uracil and leucine occurred rapidly during germ tube formation. The inhibitors of RNA synthesis, actinomycin D. cordycepin, and daunomycin prevented germination and inhibited uracil incorporation. The translational inhibitors, trichodermin, aurin tricarboxylic acid, puromycin, and cyloheximide were effective in inhibiting both germ tube formation and leucine incorporation.