Characterization of collagen orientation in human dermis by two-dimensional second-harmonic-generation polarimetry.
Characterization of collagen orientation in human dermis by two-dimensional second-harmonic-generation polarimetry.
复制标题
DOI：
10.1117/1.1644116
复制
发表时间：
2004-03
影响因子：
3.5
通讯作者：
T. Yasui;Y. Tohno;T. Araki
中科院分区：
文献类型：
--
作者：
T. Yasui;Y. Tohno;T. Araki
我们提出了一种光学探针，可以用来表征人体真皮中胶原纤维的取向。胶原蛋白的特殊探测能力来自于组织中胶原蛋白分子诱导的二次谐波（SHG）光的使用。基于胶原SHG光的概念，构建了一种探针光斑直径为15微米的反射型偏振测量系统（称为SHG偏振仪），并利用该系统对人体网状真皮层进行了测量。所得数据显示网状真皮层具有胶原纤维的近单轴取向。此外，我们展示了一种非破坏性的测量方法，可以测量乳突真皮层中胶原蛋白的取向。为了测量网状真皮层中胶原纤维取向的分布，我们将SHG偏振法扩展到一维（1-D）和二维（2-D）测量。通过宏观二维SHG偏振法，我们观察到胶原纤维的取向角和组织度随网状真皮中离散探针位置的不同而变化很大。显微1-D SHG偏振分析表明，显微区域胶原纤维的取向角增大，组织度变化较大。这些结果暗示网状真皮层具有胶原纤维的缠结结构，这与皮肤解剖检查的结果高度一致。该方法将成为监测人体真皮中胶原纤维取向的显微分布的有力工具。
We have proposed an optical probe that can be used to characterize the orientation of collagen fibers in human dermis. A specific probing ability for collagen results from the use of second-harmonic-generation (SHG) light induced by collagen molecules in the tissue. Based on the concept of collagen SHG light, a reflection-type polarization measurement system (named SHG polarimetry) with a probe light spot of 15 microm in diameter has been constructed, and the human reticular dermis has been measured using this system. Resultant data exhibit that the reticular dermis possesses approximately uniaxial orientation of the collagen fibers. Furthermore, we demonstrated a nondestructive measurement of the collagen orientation in the papillary dermis across an epidermis layer. For distribution measurement of the collagen fiber orientation in the reticular dermis, we have extended the SHG polarimetry to one- (1-D) and two-dimensional (2-D) measurement. By the macroscopic 2-D SHG polarimetry, we have observed that the orientation angle and organization degree of collagen fibers vary widely depending on the discrete probing positions in the reticular dermis. Furthermore, microscopic 1-D SHG polarimetry indicated a swell of the orientation angle and a large variance of the organization degree in the collagen fibers in the microscopic region. These results imply that the reticular dermis posses a tangled structure of collagen fibers, which is highly consistent with the result of the anatomical examination of the skin. The proposed method will be a powerful tool for monitoring the microscopic distribution of the collagen fiber orientation in the human dermis.