Dual-view microscopy with a single camera: real-time imaging of molecular orientations and calcium.
Dual-view microscopy with a single camera: real-time imaging of molecular orientations and calcium.
复制标题
带有单个相机的双视图显微镜：分子取向和钙的实时成像。
DOI：
10.1083/jcb.115.1.67
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发表时间：
1991-10
影响因子：
7.8
通讯作者：
Hayakawa, T
中科院分区：
文献类型：
--
作者：
Kinosita, K Jr;Itoh, H;Ishiwata, S;Hirano, K;Nishizaka, T;Hayakawa, T
一种新的显微镜技术，称为“W”(双视视频)显微镜，可以通过一个摄像机或肉眼同时观察一个物体的两个不同的图像。图像对可以是例如传输和荧光、不同波长的荧光、或偏振荧光的相互垂直的分量。只要简单地插入一个小型光学设备，任何视频显微镜都可以转换成双成像器。双重图像的连续出现确保了现有和未来视频显微镜的最佳时间分辨率。作为一个应用，肌动蛋白原分子在单个移动的肌动蛋白细丝中的取向已经以视频速率成像。暴露在强烈电脉冲下的细胞中也可以看到不对称的钙离子流入。
A new microscope technique, termed "W" (double view video) microscopy, enables simultaneous observation of two different images of an object through a single video camera or by eye. The image pair may, for example, be transmission and fluorescence, fluorescence at different wavelengths, or mutually perpendicular components of polarized fluorescence. Any video microscope can be converted into a dual imager by simple insertion of a small optical device. The continuous appearance of the dual image assures the best time resolution in existing and future video microscopes. As an application, orientations of actin protomers in individual, moving actin filaments have been imaged at the video rate. Asymmetric calcium influxes into a cell exposed to an intense electric pulse have also been visualized.