CREB3L4, INTS3, and SNAPAP are targets for the 1q21 amplicon frequently detected in hepatocellular carcinoma
CREB3L4, INTS3, and SNAPAP are targets for the 1q21 amplicon frequently detected in hepatocellular carcinoma
复制标题
DOI：
10.1016/j.cancergencyto.2007.09.013
复制
发表时间：
2008-01-01
影响因子：
--
通讯作者：
Okanoue, Takeshi
中科院分区：
文献类型：
--
作者：
Inagaki, Yoshikazu;Yasui, Kohichiroh;Okanoue, Takeshi
高密度单核苷酸多态性（SNP）阵列分析揭示了新的扩增在1 q21的细胞系来源于肝细胞癌（HCC）。荧光原位杂交和实时定量聚合酶链反应研究证实扩增在1 q21。在36个原发性HCC肿瘤中的32个（89%）中检测到该区域的拷贝数增加。为了确定扩增的靶点，我们检测了19个HCC细胞系中位于700-kb扩增区域内的所有26个基因的表达水平。5个基因在1 q21扩增的细胞系中过表达。其中，CREB 3L 4（cAMP反应元件结合蛋白3样4），INTS 3（整合复合物亚基3）和SNAPAP（SNAP相关蛋白）在18例HCC患者的肿瘤中显著过表达，与对应的非肿瘤组织相比。研究结果表明，CREB 3L 4，INTS 3和SNAPAP可能是扩增机制的靶点，因此可能共同或单独参与HCC的发展或进展。(C)2008年爱思唯尔公司All rights reserved.
High-density single nucleotide polymorphism (SNP) array analysis revealed novel amplification at 1q21 in cell lines derived from hepatocellular carcinomas (HCCs). Fluorescence in situ hybridization and real-time quantitative polymerase chain reaction studies verified amplification at 1q21. An increase in copy number at the region was detected in 32 of the 36 primary HCC tumors (89%). To identify the targets for amplification, we examined 19 HCC cell lines for expression levels of all 26 genes located within the 700-kb amplified region. Five genes were overexpressed in cell lines with amplification at 1q21. Among these, CREB3L4 (cAMP responsive element binding protein 3-like 4), INTS3 (integrator complex subunit 3), and SNAPAP (SNAP-associated protein) were significantly overexpressed in tumors from 18 HCC patients, compared with counterpart nontumorous tissues. The findings suggest that CREB3L4, INTS3, and SNAPAP are probable targets for the amplification mechanism and may therefore be involved, together or separately, in the development or progression of HCCs. (C) 2008 Elsevier Inc. All rights reserved.