Visualisation of stromules in transgenic wheat expressing a plastid-targeted yellow fluorescent protein
Visualisation of stromules in transgenic wheat expressing a plastid-targeted yellow fluorescent protein
复制标题
表达质体靶向黄色荧光蛋白的转基因小麦中基质的可视化
作者：
Daniel J. Shaw;J. Gray
在迄今研究过的所有多细胞植物中，胚芽是从质体延伸而来的充满基质的小管。利用农杆菌介导法，将小麦颗粒结合型淀粉合成酶I转运肽与小麦不同组织中不同类型的叶绿体上的种子体进行了嵌合，构建了针对叶绿体的增强型黄色荧光蛋白(EYFP)基因。该基因结构受控于水稻Actin1启动子，并且在所有转基因植株的所有细胞类型的质体中都检测到EYFP荧光。尽管不同组织中的球茎长度和丰度差异很大，但在所有的叶绿体类型上都观察到了球茎。叶表皮细胞中的球茎最长，达40μm，而叶肉细胞的叶绿体中仅观察到短喙状的球茎。叶和根的表皮细胞中含有最高比例的带有球茎的质体，种子发育过程中胚乳组织中的淀粉体中也含有丰富的球茎。球茎形态和分布的总体特征与烟草(Nictiana tabacum L.)和拟南芥(Arabitopsis thaliana(L.)Heynh.)
Stromules are stroma-filled tubules that extend from the plastids in all multicellular plants examined to date. To facilitate the visualisation of stromules on different plastid types in various tissues of bread wheat (Triticum aestivum L.), a chimeric gene construct encoding enhanced yellow fluorescent protein (EYFP) targeted to plastids with the transit peptide of wheat granule-bound starch synthase I was introduced by Agrobacterium-mediated transformation. The gene construct was under the control of the rice Actin1 promoter, and EYFP fluorescence was detected in plastids in all cell types throughout the transgenic plants. Stromules were observed on all plastid types, although the stromule length and abundance varied markedly in different tissues. The longest stromules (up to 40 μm) were observed in epidermal cells of leaves, whereas only short beak-like stromules were observed on chloroplasts in mesophyll cells. Epidermal cells in leaves and roots contained the highest proportion of plastids with stromules, and stromules were also abundant on amyloplasts in the endosperm tissue of developing seeds. The general features of stromule morphology and distribution were similar to those shown previously for tobacco (Nicotiana tabacum L.) and arabidopsis (Arabidopsis thaliana (L.) Heynh.).
影响因子：
56.9
作者：
Kohler, RH;Cao, J;Hanson, MR
通讯作者：
Hanson, MR
影响因子：
11.6
作者：
MCELROY, D;ZHANG, WG;WU, R
通讯作者：
WU, R