Mutations at the Saccharomyces cerevisiae SUP4 tRNA(Tyr) locus: isolation, genetic fine-structure mapping, and correlation with physical structure.
Mutations at the Saccharomyces cerevisiae SUP4 tRNA(Tyr) locus: isolation, genetic fine-structure mapping, and correlation with physical structure.
复制标题
酿酒酵母 SUP4 tRNA(Tyr) 位点突变：分离、遗传精细结构图谱以及与物理结构的相关性。
DOI：
10.1128/mcb.2.12.1501-1513.1982
复制
发表时间：
1982
影响因子：
5.3
通讯作者：
Hall,BD
中科院分区：
文献类型：
--
作者：
Kurjan,J;Hall,BD
通过分离和鉴定酿酒酵母中导致抑制功能丧失的SUP4基因突变，研究了酿酒酵母中的SUP4tRNatyrocus。大多数突变是单点突变，而大约三分之一的突变是整个基因的缺失。绘制了该基因的两张减数分裂精细结构图谱。第一种图谱技术通过测量突变对之间的重组水平，在图谱上放置了10个突变和6个up4+反密码子。第二个图谱利用了对重组频率的更定性的估计，允许定位69个突变和up4+反密码子。将这些图谱与通过DNA测序确定其核苷酸变化的34个突变的基因的物理结构进行比较(Koski等人，Cell22：415-425,1980；Kurjan等人，Cell20：701-709,1980)。这两个图谱都显示出与基因物理结构的良好相关性，尽管可以看到遗传精细结构图谱的某些特性，如标记效应和“图谱扩展”。
TheSUP4tRNATyrlocus in Saccharomyces cerevisiae has been studied by the isolation and characterization of mutations at theSUP4gene which result in the loss of suppressor function. Most of the mutations act as single-site mutations, whereas about a third of the mutations are deletions of the entire gene. Two meiotic fine-structure maps of the gene were made. The first mapping technique placed 10 mutations plus thesup4+anticodon on a map by a measurement of levels of recombination between pairs of mutations. The second map utilized a more qualitative estimate of recombination frequency, allowing 69 mutations and thesup4+anticodon to be mapped. The maps were compared with the physical structure of the gene for the 34 mutations whose nucleotide alteration has been determined by DNA sequencing (Koski et al., Cell22:415–425, 1980; Kurjan et al., Cell20:701–709, 1980). Both maps show a good correlation with the physical structure of the gene, even though certain properties of genetic fine-structure maps, such as marker effects and “map expansion,” were seen.