Phenotypic traits of Burkholderia spp. associated with ecological adaptation and plant-host interaction.
Phenotypic traits of Burkholderia spp. associated with ecological adaptation and plant-host interaction.
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DOI：
10.1016/j.micres.2020.126451
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发表时间：
2020-02
影响因子：
6.7
通讯作者：
Karent J Romero-Gutiérrez;M. N. Dourado;L. M. Garrido;L. R. Olchanheski;E. T. Mano;F. Dini‐Andreote;M. Valvano;W. L. Araújo
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文献类型：
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作者：
Karent J Romero-Gutiérrez;M. N. Dourado;L. M. Garrido;L. R. Olchanheski;E. T. Mano;F. Dini‐Andreote;M. Valvano;W. L. Araújo
伯克霍尔德菌种有不同的生活方式，与植物和动物建立互利或致病的联系。这些细菌生态行为的变化可能取决于遗传变异对生态位适应的反应。本文对15株不同环境分离的伯克霍尔德氏菌进行了遗传和表型特征的研究。通过多位点序列分析（MLSA），这些菌株可分为6个不同的类群。MLSA簇与菌株抗生素敏感性无关，但与细菌产生抗菌化合物和控制兰花坏死的能力有关。此外，B。将一株互利共生细菌TC3.4.2R3接种到兰花植株上，通过分析植物的反应和植物体内细菌的氧化胁迫反应来评价其与寄主的相互作用。TC3.4.2R3通过增加其自身的生长速率和通过对由植物引起的氧化胁迫的差异基因调节来响应植物定殖，同时减少植物的膜脂质过氧化。细菌对氧化应激的反应通过细菌暴露于除草剂百草枯来重现。我们认为，伯克霍尔德氏菌种的能力，成功地建立在根际与遗传变异，而与抗生素耐药性相关的性状更有可能被归类为菌株特异性。
Burkholderiaspecies have different lifestyles establishing mutualist or pathogenic associations with plants and animals. Changes in the ecological behavior of these bacteria may depend on genetic variations in response to niche adaptation. Here, we studied 15Burkholderiastrains isolated from different environments with respect to genetic and phenotypic traits. By Multilocus Sequence Analysis (MLSA) these isolates fell into 6 distinct groups. MLSA clusters did not correlate with strain antibiotic sensitivity, but with the bacterial ability to produce antimicrobial compounds and control orchid necrosis. Further, theB. seminalisstrain TC3.4.2R3, a mutualistic bacterium, was inoculated into orchid plants and the interaction with the host was evaluated by analyzing the plant response and the bacterial oxidative stress responsein planta. TC3.4.2R3 responded to plant colonization by increasing its own growth rate and by differential gene regulation upon oxidative stress caused by the plant, while reducing the plant's membrane lipid peroxidation. The bacterial responses to oxidative stress were recapitulated by bacterial exposure to the herbicide paraquat. We suggest that the ability ofBurkholderiaspecies to successfully establish in the rhizosphere correlates with genetic variation, whereas traits associated with antibiotic resistance are more likely to be categorized as strain specific.