Phenol-Rich Feijoa sellowiana (Pineapple Guava) Extracts Protect Human Red Blood Cells from Mercury-Induced Cellular Toxicity
Phenol-Rich Feijoa sellowiana (Pineapple Guava) Extracts Protect Human Red Blood Cells from Mercury-Induced Cellular Toxicity
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富含苯酚的 Feijoa sellowiana（菠萝番石榴）提取物可保护人类红细胞免受汞引起的细胞毒性
DOI：
10.3390/antiox8070220
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发表时间：
2019
期刊：
影响因子：
7
通讯作者：
C. Manna
中科院分区：
文献类型：
--
作者：
Fabiana Tortora;Rosaria Notariale;V. Maresca;Katrina V Good;S. Sorbo;A. Basile;M. Piscopo;C. Manna
植物多酚具有广泛的抗氧化特性，是对抗环境氧化应激源（包括汞）的非常有效的试剂。这种重金属不可逆地结合巯基，螯合内源性抗氧化剂，如谷胱甘肽。由于从肾脏到心血管疾病等许多严重的下游影响，食源性汞的发病率增加令人担忧。因此，可能有益的特性Feijoa sellowiana对汞毒性进行了测试，使用完整的人红细胞（RBC）孵育的存在下，氯化汞。在这里，我们表明，从Feijoa sellowiana果实中提取的富含苯酚（10-200 µg/mL）的提取物可以有效地防止汞诱导的毒性和氧化应激。果皮和果肉提取物都能对抗汞处理引起的红细胞氧化应激和巯基减少。尽管如此，与果肉相比，果皮提取物具有更大的保护作用，尽管对于所分析的不同标记物具有不同程度的保护作用，这至少部分是由于果皮中多酚的更大比例和多样性。此外，Fejioa sellowiana提取物还可以防止汞诱导的形态变化，已知这种变化可以增强这些细胞的促凝血活性。这些新发现为基于Fejioa sellowiana的功能性食品在预防和对抗汞相关疾病方面的药理学用途提供了生物化学基础。
Plant polyphenols, with broadly known antioxidant properties, represent very effective agents against environmental oxidative stressors, including mercury. This heavy metal irreversibly binds thiol groups, sequestering endogenous antioxidants, such as glutathione. Increased incidence of food-derived mercury is cause for concern, given the many severe downstream effects, ranging from kidney to cardiovascular diseases. Therefore, the possible beneficial properties of Feijoa sellowiana against mercury toxicity were tested using intact human red blood cells (RBC) incubated in the presence of HgCl2. Here, we show that phenol-rich (10–200 µg/mL) extracts from the Feijoa sellowiana fruit potently protect against mercury-induced toxicity and oxidative stress. Peel and pulp extracts are both able to counteract the oxidative stress and thiol decrease induced in RBC by mercury treatment. Nonetheless, the peel extract had a greater protective effect compared to the pulp, although to a different extent for the different markers analyzed, which is at least partially due to the greater proportion and diversity of polyphenols in the peel. Furthermore, Fejioa sellowiana extracts also prevent mercury-induced morphological changes, which are known to enhance the pro-coagulant activity of these cells. These novel findings provide biochemical bases for the pharmacological use of Fejioa sellowiana-based functional foods in preventing and combating mercury-related illnesses.
影响因子：
7.4
作者：
VANDENBERG, JJM;DENKAMP, JAF;KUYPERS, FA
通讯作者：
KUYPERS, FA