THE C-FMS PROTO-ONCOGENE PRODUCT IS RELATED TO THE RECEPTOR FOR THE MONONUCLEAR PHAGOCYTE GROWTH-FACTOR, CSF-1
THE C-FMS PROTO-ONCOGENE PRODUCT IS RELATED TO THE RECEPTOR FOR THE MONONUCLEAR PHAGOCYTE GROWTH-FACTOR, CSF-1
复制标题
DOI：
10.1016/s0092-8674(85)80047-7
复制
发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
STANLEY, ER
中科院分区：
文献类型：
--
作者：
SHERR, CJ;RETTENMIER, CW;STANLEY, ER
猫科动物 c-fms 原癌基因产物是一种 170 kd [千道尔顿] 糖蛋白，具有相关的酪氨酸激酶活性。这种糖蛋白在来自腹膜炎症渗出物和脾脏的成熟猫巨噬细胞上表达。小鼠集落刺激因子 CSF-1（单核吞噬细胞生长因子）的受体仅限于单核吞噬细胞谱系的细胞，是一种具有相关酪氨酸激酶的 165 kd 糖蛋白。针对重组 v-fms 编码多肽的兔抗血清沉淀了猫 c-fms 产物，并与来自小鼠巨噬细胞的 165 kd 糖蛋白发生特异性交叉反应。鼠 c-fms 基因的这种推定产物在免疫复合物中表现出相关的酪氨酸激酶活性，特异性结合鼠 CSF-1，并且在生长因子存在的情况下，在膜制剂中的 Tyr 上被磷酸化。因此，鼠c-fms原癌基因产物和CSF-1受体是相关的，并且可能是相同的分子。
The feline c-fms proto-oncogene product is a 170 kd [kilodalton] glycoprotein with associated tyrosine kinase activity. This glycoprotein was expressed on mature cat macrophages from peritoneal inflammatory exudates and spleen. The receptor for the murine colony-stimulating factor, CSF-1 [mononuclear phagocyte growth factor], is restricted to cells of the mononuclear phagocytic lineage and is a 165 kd glycoprotein with an associated tyrosine kinase. Rabbit antisera to a recombinant v-fms-coded polypeptide precipitated the feline c-fms product and specifically cross-reacted with a 165 kd glycoprotein from mouse macrophages. This putative product of the murine c-fms gene exhibited an associated tyrosine kinase activity in immune complexes, specifically bound murine CSF-1, and, in the presence of the growth factor, was phosphorylated on Tyr in membrane preparations. The murine c-fms proto-oncogene product and the CSF-1 receptor are therefore related, and possibly identical, molecules.