Host transcriptional signatures as predictive markers of infection in children.
Host transcriptional signatures as predictive markers of infection in children.
复制标题
宿主转录特征作为儿童感染的预测标记。
DOI：
10.1097/qco.0000000000000750
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发表时间：
2021-10-01
影响因子：
3.9
通讯作者：
Ramilo O
中科院分区：
文献类型：
--
作者：
Mejias A;Cohen S;Glowinski R;Ramilo O
分析宿主对感染的转录反应已被证明是标准的直接病原体检测的另一种诊断策略。本文综述了血液和粘膜转录组分析在儿童病毒和细菌感染诊断和治疗中的应用价值。多年来，研究已经证实了一个概念，即来自患有传染病的儿童的RNA转录谱携带一种可以定性和定量测量的病原体特异性生物特征。这些生物特征可以转化为具有生物学意义的背景：1)改善患者诊断，如结核病儿童、鼻病毒感染儿童、发热婴儿和肺炎儿童；2)了解疾病的发病机制（如先天性巨细胞病毒），3)根据临床严重程度（如RSV）对患者进行客观分类。宿主RNA转录免疫反应的全球评估提高了我们对临床环境中宿主-病原体相互作用的理解。它显示了在我们目前的诊断工具不足的临床情况下使用的潜力，指导患有传染病的儿童的诊断和分类。
Analyses of the host transcriptional response to infection has proved to be an alternative diagnostic strategy to standard direct pathogen detection. This review summarizes the value of applying blood and mucosal transcriptome analyses for the diagnosis and management of children with viral and bacterial infections. Over the years, studies have validated the concept that RNA transcriptional profiles derived from children with infectious diseases carry a pathogen-specific biosignature that can be qualitatively and quantitively measured. These biosignatures can be translated into a biologically meaningful context to: 1) improve patient diagnosis, as seen in children with tuberculosis, rhinovirus infections, febrile infants, and children with pneumonia; 2) to understand disease pathogenesis (i.e. congenital CMV), and 3) to objectively classify patients according to clinical severity (i.e. RSV). The global assessment of host RNA transcriptional immune responses has improved our understanding of the host-pathogen interactions in the clinical setting. It has shown the potential to be used in clinical situations where our current diagnostic tools are inadequate, guiding the diagnosis and classification of children with infectious diseases.