ADAM7, a member of the ADAM (a disintegrin and metalloprotease) gene family is specifically expressed in the mouse anterior pituitary and epididymis
ADAM7, a member of the ADAM (a disintegrin and metalloprotease) gene family is specifically expressed in the mouse anterior pituitary and epididymis
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DOI：
10.1210/en.138.10.4262
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发表时间：
1997-10-01
期刊：
影响因子：
4.8
通讯作者：
Hsia, N
中科院分区：
文献类型：
--
作者：
Cornwall, GA;Hsia, N
附睾中精子的成熟是一个复杂的过程，需要附睾上皮的积极参与。阐明附睾在成熟过程中的作用的主要焦点是附睾分泌蛋白及其与精子相互作用的研究。迄今为止，关于附睾上皮细胞表面蛋白的信息很少，这些蛋白也可能在附睾功能中发挥重要作用。通过消减杂交方法鉴定附睾头中特异性表达的基因，鉴定出 ADAM（解整合素和金属蛋白酶）蛋白家族成员的小鼠同源物。这个快速增长的基因家族编码具有假定的粘附和蛋白酶结构域的细胞表面蛋白。 Northern 印迹分析表明，小鼠 ADAM 基因（称为 ADAM7）在附睾头状区域和垂体前叶促性腺激素中表达，而在所检查的 26 个其他组织中未检测到表达。此外，原位杂交实验表明，ADAM7 信使 RNA (mRNA) 在附睾近端上皮中表现出顶端定位，这可能与附睾近端区域独特存在的不寻常的稀疏颗粒内质网有关，但尚未确定其功能。激素、手术和遗传策略表明，ADAM7 基因表达以区域依赖性方式需要雄激素和睾丸因子才能表达。有趣的是，ADAM7 mRNA 的顶端定位取决于完整的睾丸，因为对维持睾酮的去势小鼠的近端附睾进行原位杂交分析，未显示 ADAM7 mRNA 的顶端定位。最后，染色体作图表明，ADAM7 基因定位于小鼠 14 号染色体的中央区域，距离生育素 β 基因座约 4-5 cM，该基因座编码另一种生殖特异性 ADAM 蛋白。
The maturation of spermatozoa in the epididymis is a complex process that requires the active involvement of the epididymal epithelium. The primary focus toward elucidating the role of the epididymis in the maturation process has been the study of epididymal secretory proteins and their interaction with spermatozoa. To date there is a paucity of information regarding epididymal epithelial cell surface proteins, which may also play important roles in epididymal function. Through a subtractive hybridization approach to identify genes specifically expressed in the caput epididymidis, the mouse homologue of a member of the ADAM (a disintegrin and metalloprotease) family of proteins was identified. This rapidly growing gene family encodes cell surface proteins that possess putative adhesion and protease domains. Northern blot analyses demonstrated that the mouse ADAM gene, termed ADAM7, is expressed in the caput region of the epididymis and in the anterior pituitary gonadotropes with no detectable expression in the twenty-six other tissues examined. Furthermore, in situ hybridization experiments revealed that the ADAM7 messenger RNA (mRNA) exhibited an apical localization within the proximal caput epididymal epithelium that may correlate with an unusual sparsely granulated endoplasmic reticulum uniquely present in the proximal region of the epididymidis and to which no known function has been ascribed. Hormonal, surgical, and genetic strategies demonstrated that ADAM7 gene expression requires, in a region-dependent manner, androgens as well as testicular factors for expression. Interestingly, the epical localization of ADAM7 mRNA is dependent upon an intact testis, because in situ hybridization analyses of the proximal caput epididymidis from a testosterone maintained castrate mouse did not show the apical localization of ADAM7 mRNA. Finally, chromosomal mapping demonstrated that the ADAM7 gene maps to the central region of mouse Chromosome 14, approximately 4-5 cM distal from the fertilin beta locus, which encodes another reproductive-specific ADAM protein.