gon-14 functions with class B and class C synthetic multivulva genes to control larval growth in Caenorhabditis elegans
gon-14 functions with class B and class C synthetic multivulva genes to control larval growth in Caenorhabditis elegans
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DOI：
10.1534/genetics.105.048751
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发表时间：
2006-02-01
期刊：
影响因子：
3.3
通讯作者：
Kimble, J
中科院分区：
文献类型：
--
作者：
Chesney, MA;Kidd, AR;Kimble, J
先前的研究表明，线虫 gon-14 是性腺发生所必需的。在这里，我们报告 gon-14 编码的蛋白质与 LIN-15B（一种 B 类 synMuv 蛋白质）相似。 GON-14 的一个广泛区域包含与 hAT 超家族转座酶相似的序列块，但关键残基不保守，表明存在较远的关系。 GON-14 还包含一个推定的 THAP DNA 结合域。拯救性 gon-14::GON-14::VENUS 报告基因在发育过程中广泛表达并定位于细胞核。严重功能丧失和预测无效的 gon-14 等位基因具有多效性缺陷，包括多外阴 (Muv) 缺陷和温度敏感的幼虫停滞。尽管 synMuv 突变不会增强 gon-14 Muv 缺陷，但 gon-14 与 B 类和 C 类 synMuv 基因（包括 lin-35/Rb、let-418/Mi-2 beta 和 trr-1/TRRAP）存在遗传相互作用。 gon-14； synMuv 双突变体在支持各自单突变体发育成成年的条件下生长时，作为幼虫被捕集。 gon-14 幼虫停滞会因编码 SET 结构域蛋白的 mes-2/E(z)、mes-6/ESC 或 mes-4 的缺失而受到抑制。此外，gon-14 影响 pgl-1 和 lag-2 的表达，这两个基因受 svnMuv 基因调节。 Tore 认为 gon-14 与 B 类和 C 类 synMuv 基因一起发挥作用，促进幼虫生长，部分是通过拮抗 MES-2,3,6/ESC-E(z) 和 MES-4 来实现的。
Previous work showed that C. elegans gon-14 is required for gonadogenesis. Here we report that gon-14 encodes a protein with similarity to LIN-15B, a class B synMuv protein. An extensive region of GON-14 contains blocks of sequence similarity to transposases of the hAT superfamily, but key residues are not conserved, suggesting a distant relationship. GON-14 also contains a putative THAP DNA-binding domain. A rescuing gon-14::GON-14::VENUS reporter is broadly expressed during development and localizes to the nucleus. Strong loss-of-function and predicted null gon-14 alleles have pleiotropic defects, including multivulval (Muv) defects and temperature-sensitive larval arrest. Although the gon-14 Muv defect is not enhanced by synMuv mutations, gon-14 interacts genetically with class B and class C synMuv genes, including lin-35/Rb, let-418/Mi-2 beta, and trr-1/TRRAP. The gon-14; synMuv double mutants arrest as larvae when grown under conditions supporting development to adulthood for the respective single mutants. The gon-14 larval arrest is suppressed by loss of mes-2/E(z), mes-6/ESC, or mes-4, which encodes a SET domain protein. Additionally, gon-14 affects expression of pgl-1 and lag-2, two genes regulated by the svnMuv genes. Tore suggest that gon-14 functions with class B and class C synMuv genes to promote larval growth, in part by antagonizing MES-2,3,6/ESC-E(z) and MES-4.