Increased nitrogen-use efficiency in transgenic rice plants over-expressing a nitrogen-responsive early nodulin gene identified from rice expression profiling
Increased nitrogen-use efficiency in transgenic rice plants over-expressing a nitrogen-responsive early nodulin gene identified from rice expression profiling
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DOI：
10.1111/j.1365-3040.2009.02032.x
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发表时间：
2009-12-01
影响因子：
7.3
通讯作者：
Rothstein, Steven J.
中科院分区：
文献类型：
--
作者：
Bi, Yong-Mei;Kant, Surya;Rothstein, Steven J.
开发提高氮利用效率（NUE）的遗传品种对于可持续农业至关重要。在这项研究中，我们开发了一种水稻生长系统，其中氮是生长限制因子，并通过全基因组转录分析方法鉴定了氮响应基因。选择一些基因通过转基因方法在 NUE 中测试其功能。对 NUE 产生积极影响的一个例子是早期结节蛋白基因 OsENOD93-1。 OsENOD93-1 基因对氮诱导和氮减少均有显着反应。过度表达 OsENOD93-1 基因的转基因水稻植物增加了地上部干生物量和种子产量。该 OsENOD93-1 基因在野生型 (WT) 植物的根部高水平表达，其蛋白质产物位于线粒体中。转基因植物根部积累了更高浓度的总氨基酸和总氮。在转基因植物中检测到木质部汁液中氨基酸浓度较高，尤其是在氮胁迫下。原位杂交显示 OsENOD93-1 在维管束以及表皮和内皮中表达。这项工作表明，转录分析与转基因验证方法相结合是基因发现的有效策略。从这项研究中获得的知识可以应用于其他重要作物。
Development of genetic varieties with improved nitrogen-use efficiency (NUE) is essential for sustainable agriculture. In this study, we developed a growth system for rice wherein N was the growth-limiting factor, and identified N-responsive genes by a whole genome transcriptional profiling approach. Some genes were selected to test their functionality in NUE by a transgenic approach. One such example with positive effects on NUE is an early nodulin gene OsENOD93-1. This OsENOD93-1 gene responded significantly to both N induction and N reduction. Transgenic rice plants over-expressing the OsENOD93-1 gene had increased shoot dry biomass and seed yield. This OsENOD93-1 gene was expressed at high levels in roots of wild-type (WT) plants, and its protein product was localized in mitochondria. Transgenic plants accumulated higher concentrations of total amino acids and total N in roots. A higher concentration of amino acids in xylem sap was detected in transgenic plants, especially under N stress. In situ hybridization revealed that OsENOD93-1 is expressed in vascular bundles, as well as in epidermis and endodermis. This work demonstrates that transcriptional profiling, coupled with a transgenic validation approach, is an effective strategy for gene discovery. The knowledge gained from this study could be applied to other important crops.