PCR-based determination of internal transcribed spacer (ITS) regions of ribosomal DNA of giant intestinal fluke, Fasciolopsis buski (Lankester, 1857) Looss, 1899
PCR-based determination of internal transcribed spacer (ITS) regions of ribosomal DNA of giant intestinal fluke, Fasciolopsis buski (Lankester, 1857) Looss, 1899
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DOI：
10.1007/s00436-007-0680-y
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发表时间：
2007-11-01
影响因子：
2
通讯作者：
Bandyopadhyay, S.
中科院分区：
文献类型：
--
作者：
Prasad, P. K.;Tandon, V.;Bandyopadhyay, S.
猪片吸虫是南亚和东南亚猪的一种人畜共患病的肠道吸虫，在印度东北部地区普遍流行。采集自该地区不同地区的吸虫种群在形态上表现出多样性。我们研究的主要目的是利用核糖体DNA簇(RDNA)提供寄生虫的分子特征，以补充形态标准，核糖体DNA簇的两侧有更保守的内部转录间隔区(ITS)。我们在这里描述了从阿萨姆邦地区的猪宿主中收集到的寄生虫的ITS序列。卵和成虫的ITS序列在长度和组成上都是相同的。在系统发育上，布氏吸虫与片形虫科的其他成员非常相似，在比对中显示出显著的期望价值。这些结果证实了ITS序列不是阶段性的，在吸虫发育的不同阶段都是保守的，因此可以作为物种标记。
Fasciolopsis buski, the zoonotic intestinal flukes of pigs in South and Southeast Asia, is commonly prevalent in regions across Northeast India. Populations of the fluke collected from different parts of the region exhibit variations in morphology. The main objective of our study was to provide molecular characterization of the parasite so as to supplement morphological criteria, using ribosomal DNA cluster (rDNA), which is flanked by more conserved internal transcribed spacer (ITS) regions. We describe herein the ITS sequences of the parasite collected from swine hosts of Assam region. The ITS sequences of both egg and adult origins were found to be identical in length and composition. Phylogenetically, F. buski resembles closely the other members of family Fasciolidae, showing significant expectation value in the alignment. The results corroborate that the ITS sequences are not stage specific and are conserved through different stages of development of the fluke, and thus could be used as species markers.