Granular Honeycombs Composed of Carbonate Apatite, Hydroxyapatite, and β-Tricalcium Phosphate as Bone Graft Substitutes: Effects of Composition on Bone Formation and Maturation
Granular Honeycombs Composed of Carbonate Apatite, Hydroxyapatite, and β-Tricalcium Phosphate as Bone Graft Substitutes: Effects of Composition on Bone Formation and Maturation
复制标题
DOI：
10.1021/acsabm.0c00060
复制
发表时间：
2020-03-16
影响因子：
4.7
通讯作者：
Ishikawa, Kunio
中科院分区：
文献类型：
--
作者：
Hayashi, Koichiro;Kishida, Ryo;Ishikawa, Kunio
材料成分和多孔结构是新骨形成和成熟以及新骨替代材料的重要因素。传统的骨移植材料由于其大孔结构的复杂性通常不适合骨生成，这会干扰材料中与骨重塑和血管生成相关的细胞的渗透。在本研究中，制备了具有均匀尺寸大孔（类似于115μm）的碳酸盐磷灰石（CO（3）Ap）、羟基磷灰石（HAp）和β-磷酸三钙（TCP）蜂窝状颗粒（HCG）。这些HCG大孔以规则的方式排列并直接渗透到颗粒中。它们被植入兔子股骨缺损处以进行进一步评估。在CO(3)Ap HCG植入组中，移植后4周，CO(3)Ap HCG大孔内形成成熟骨，并且在12周时大部分CO(3)Ap HCG被新骨替代。相比之下，在β-TCP HCG植入组中，在β-TCP HCG消失后的区域中并不总是形成新骨，并且即使在12周后，β-TCP HCG大孔内仍存在未成熟骨。 HAp HCG 没有被再吸收，其大孔充满了未成熟的骨。与HAp和β-TCP HCG植入组相比，CO(3)Ap HCG植入组的成熟骨面积在4周时分别增加3.3倍和1.6倍，在12周时分别增加2.2倍和1.7倍。此外，CO(3)Ap、HAp和β-TCP HCG的骨成熟度在4周时分别为100%、34%和64%，在12周时分别为100%、54%和69%。因此，HCG 的组成影响骨的形成和成熟。
Material composition and porous structure are important factors in the formation and maturation of newly formed bone and replacement of materials by new bone. Conventional bone graft materials often lack suitability for bone generation because of the complexity of their macroporous structures, which can interfere with the penetration of cells related to bone remodeling and angiogenesis in the materials. In the present study, carbonate apatite (CO(3)Ap), hydroxyapatite (HAp), and beta-tricalcium phosphate (TCP) honeycomb granules (HCGs) with uniformly sized macropores (similar to 115 mu m) were fabricated. These HCG macropores were arranged in a regular fashion and penetrated straight into the granules. They were implanted into a rabbit femur defect for further evaluation. In the CO(3)Ap HCG implantation group, mature bone formed within CO(3)Ap HCG macropores by 4 weeks after grafting, and a large portion of CO(3)Ap HCGs was replaced by new bone at 12 weeks. By contrast, in the beta-TCP HCG implantation group, new bone was not always formed in the regions after beta-TCP HCG disappearance, and immature bone was present within beta-TCP HCG macropores even after 12 weeks. HAp HCGs were not resorbed, and their macropores were filled with immature bone. The area of mature bone in the CO(3)Ap HCG implantation group was 3.3 and 1.6 times higher at 4 weeks and 2.2 and 1.7 times higher at 12 weeks compared with the HAp and beta-TCP HCG implantation groups, respectively. Furthermore, the degrees of bone maturation for CO(3)Ap, HAp, and beta-TCP HCGs were 100, 34, and 64% at 4 weeks, and 100, 54, and 69% at 12 weeks, respectively. Thus, the composition of the HCGs affected bone formation and maturation.