Escape of mouse mastocytoma P815 after nearly complete rejection is due to antigen-loss variants rather than immunosuppression.
Escape of mouse mastocytoma P815 after nearly complete rejection is due to antigen-loss variants rather than immunosuppression.
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DOI：
10.1084/jem.157.3.1040
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发表时间：
1983-03-01
期刊：
影响因子：
--
通讯作者：
Boon T
中科院分区：
文献类型：
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作者：
Uyttenhove C;Maryanski J;Boon T
尽管肥大细胞瘤P815在同基因小鼠中经常经历几乎完全的排斥，但肿瘤细胞几乎总是逃逸形成进行性肿瘤。我们发现，这不是由于免疫抑制状态的建立，因为基因标记的P815细胞，被注射到发生肿瘤逃逸的小鼠中，很容易被排斥。用抗P815溶细胞性T淋巴细胞（CTL）克隆分析逃逸的肿瘤细胞群体显示存在稳定的抗性变体。使用逃逸人群中发现的或在体外用CTL克隆选择的抗原丢失变体，我们能够定义四种不同的肿瘤相关抗原特异性，每种抗原特异性由特定的CTL克隆识别。其中一种特异性在所有逃逸的肿瘤细胞中缺失，另一种特异性在其中一些细胞中丢失。
Even though mastocytoma P815 often undergoes a nearly complete rejection in syngeneic mice, the tumor cells almost always escape to form progressive tumors. We found that this was not due to the establishment of an immunosuppressed state because genetically marked P815 cells, that were injected in mice where tumor escape was occurring, were readily rejected. An analysis of escaping tumor cell populations with anti-P815 cytolytic T lymphocyte (CTL) clones showed the presence of stable resistant variants. Using antigen-loss variants found in escaping populations or selected in vitro with CTL clones, we were able to define four different tumor-associated antigenic specificities, each recognized by a specific CTL clone. One of these specificities was absent from all escaping tumor cells and another had been lost by some of them.