Natural variation and genetic analysis of the tiller angle gene MsTAC1 in Miscanthussinensis
Natural variation and genetic analysis of the tiller angle gene MsTAC1 in Miscanthussinensis
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DOI：
10.1007/s00425-014-2070-x
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发表时间：
2014-04
期刊：
影响因子：
4.3
通讯作者：
Hua Zhao;Zexun Huai;Yingjie Xiao;Xiaohua Wang;Jiangyan Yu;G. Ding;Junhua Peng
中科院分区：
文献类型：
--
作者：
Hua Zhao;Zexun Huai;Yingjie Xiao;Xiaohua Wang;Jiangyan Yu;G. Ding;Junhua Peng
生物量产量是芒草育种中理想能源作物的一个重要目标性状。分蘖角度是植物结构的关键性状，因为它决定种植密度，并通过影响光合作用效率进一步影响生物量产量。TAC1是分别参与水稻和玉米分蘖和叶角控制的主要基因，已被广泛研究。在中国不同地区收集的33份芒草材料和芒草×巨花的1个基因型中对该基因MsTAC1的核苷酸变异进行了研究。 1,874 bp 的 MsTAC1 基因内共有 136 个位点，包括 129 个单碱基替换和 7 个 InDels。 MsTAC1的遗传多样性具有高核苷酸多样性（π值）和高杂合性的特点。聚类分析表明33M.sinensis种质的系统发育树与其起源地相关。 中性测试表明，对于材料中MsTAC1基因的编码区和非编码区变异，没有强烈的选择压力。在2009年和2010年的前两个生长年中，对芒草的分蘖角度和其他五个性状进行了表型评估。方差分析显示所检查的性状存在显着的表型变异。使用 246 个标记进行关联分析，检测到 1 年或 2 年内与所有测试性状相关的 88 个位点，其中 88 个位点中的 11 个可在一年内重现，因此可靠。这些关联表明MsTAC1的变异影响分蘖角度、分蘖数和生物量产量的表型值，表明MsTAC1的等位基因变异影响多种性状，并证明了其在芒草育种计划中的重要性。
Biomass yield is an important target trait inMiscanthusbreeding for desirable energy crops. Tiller angle is a key trait of plant architecture because it determines planting density and further influences biomass yield through affecting photosynthesis efficiency.TAC1, a major gene involved in tiller and leaf angle control in rice and maize, respectively, has been extensively studied. Nucleotide variation at this gene,MsTAC1, was investigated in 33Miscanthus sinensisaccessions collected from different areas in China, and one genotype ofMiscanthus×giganteus. A total of 136 loci, including 129 single base substitutions and seven InDels, occurred within theMsTAC1gene of 1,874 bp. The genetic diversity atMsTAC1is characterized by high nucleotide diversity (πvalue) and high heterozygosity. Clustering analysis indicated that the phylogenetic tree of the 33M.sinensisaccessions was correlated with their geographical sites of origin. The neutrality test revealed no strong selection pressure on coding and non-coding region variations of theMsTAC1gene in the accessions. Phenotype evaluations were conducted for tiller angle and five other traits in theMiscanthuspanels in the first two growth years of 2009 and 2010. Analysis of variance showed significant phenotypic variations in the examined traits. Association analysis using 246 markers detected 88 loci associated with all the test traits in either 1 or 2 years, and 11 of the 88 were year reproducible and thus reliable. These associations indicate that the variation ofMsTAC1affects the phenotypic value of the tiller angle, tiller number and biomass yield, suggesting that allelic variation inMsTAC1affects multiple traits and demonstrates its significance inMiscanthusbreeding programs.