Mass production of the ginsenoside Rg3(S) through the combinative use of two glycoside hydrolases
Mass production of the ginsenoside Rg3(S) through the combinative use of two glycoside hydrolases
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DOI：
10.1016/j.foodchem.2013.04.012
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发表时间：
2013-11-15
期刊：
影响因子：
8.8
通讯作者：
Im, Wan-Taek
中科院分区：
文献类型：
--
作者：
Kim, Jin-Kwang;Cui, Chang-Hao;Im, Wan-Taek
作为韩国红参提取物的特殊成分之一的人参皂苷Rg（3）（S）已知具有抗癌、抗转移和抗肥胖作用。为了获得高特异性、高收率和高纯度的β-Rg（3）（S），开发了一种酶促生物转化方法。使用两种糖苷水解酶（BglBX 10和Abf 22 -3）来生产100 g单位的Rg（3）（S）。转化反应使用Abf 22 -3将Rb Rc转化为Rd，然后使用BglBX 10将Rb-1和Rd转化为Rg（3）（S）。在10 μ l发酵罐中，在pH6.0和37 ℃下进行24小时，高浓度为50 mg/ml的从人参根获得的纯化的人参混合物。最后，从250 g的根提取物中产生144 g的Rg（3）（S），具有78 +/- 1.2%的色谱纯度。这些结果表明，这种酶法将是有用的，用于功能食品和医药工业的制备Rg（3）（S）。(C)2013爱思唯尔有限公司保留所有权利。
The ginsenoside Rg(3)(S), which is one of the exceptional components of Korean red ginseng extract, has been known to have anti-cancer, anti-metastatic, and anti-obesity effects. An enzymatic bioconversion method was developed to obtain the ginsenoside Rg(3)(S) with a high specificity, yield, and purity. Two glycoside hydrolases (BglBX10 and Abf22-3) were employed to produce Rg(3)(S) as a 100 g unit. The conversion reaction transformed ginsenoside Rc to Rd using Abf22-3, followed by Rb-1 and Rd to Rg(3)(S), using BglBX10. It was performed in a 10 Liar fermenter at pH 6.0 and 37 degrees C for 24 h, with a high concentration of 50 mg/ml of purified ginsenoside mixture obtained from ginseng roots. Finally, 144 g of Rg(3)(S) was produced from 250 g of root extract with 78 +/- 1.2% chromatographic purity. These results suggest that this enzymatic method would be useful in the preparation of ginsenoside Rg(3)(S) for the functional food and pharmaceutical industries. (C) 2013 Elsevier Ltd. All rights reserved.