EFFECT OF FRUCTOSE AND OTHER CARBOHYDRATES ON THE SURFACE-PROPERTIES, LIPOTEICHOIC ACID PRODUCTION, AND EXTRACELLULAR PROTEINS OF STREPTOCOCCUS-MUTANS INGBRITT GROWN IN CONTINUOUS CULTURE
EFFECT OF FRUCTOSE AND OTHER CARBOHYDRATES ON THE SURFACE-PROPERTIES, LIPOTEICHOIC ACID PRODUCTION, AND EXTRACELLULAR PROTEINS OF STREPTOCOCCUS-MUTANS INGBRITT GROWN IN CONTINUOUS CULTURE
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DOI：
10.1128/iai.31.1.78-87.1981
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发表时间：
1981-01-01
影响因子：
3.1
通讯作者：
WICKEN, AJ
中科院分区：
文献类型：
--
作者：
HARDY, L;JACQUES, NA;WICKEN, AJ
S. mutans Ingbritt 在恒化器中以 0.5% 果糖或 0.5% 山梨糖醇的指定稀释率以及 0.5% 果糖的指定 pH 值生长。细胞产量受碳水化合物来源和 pH 值影响，0.5% 果糖中 pH 5.5 时产量最低。果糖生长的细胞比相应的葡萄糖生长的细胞更容易被胞壁质酶裂解，但相应细胞壁制剂的裂解敏感性或壁裂解物与变形链球菌英布里特抗血清的血清学反应性没有显着差异。在果糖和山梨糖醇中的高稀释率以及果糖的高pH值下获得最大量的细胞脂磷壁酸。通过火箭免疫电泳，在低稀释率下发现了最大量的细胞外脂磷壁酸，并确定了生长条件对这些成分的影响。分批培养的培养物在细胞对胞壁质酶裂解的敏感性方面存在基因型变异。分批培养中果糖和山梨糖醇对脂磷壁酸产生的增强作用在变形链球菌血清型 c 的代表性菌株中并不相同，并且在不同变形链球菌血清型的代表性菌株中果糖的效果也不一致。
S. mutans Ingbritt was grown in a chemostat at destined dilution rates in either 0.5% fructose or 0.5% sorbitol and at destined pH values in 0.5% fructose. Cell yield was affected by the carbohydrate source and the pH, with the lowest yield being at pH 5.5 in 0.5% fructose. Fructose-grown cells showed greater susceptibility to lysis by a muramidase than the corresponding glucose-grown cells, but there were no marked differences in the lytic susceptibilities of the corresponding cell wall preparations or in the serological reactivities of wall lysates with antiserum to S. mutans Ingbritt. The greatest amounts of cellular lipoteichoic acid were obtained at high dilution rates in fructose and sorbitol, as well as at high pH values in fructose. The greatest amounts of extracellular lipoteichoic acid were found at low dilution rates, by rocket immunoelectrophoresis, and the effects of growth conditions on these components were determined. There was genotypic variation for batch-grown cultures in the susceptibility of cells to lysis by a muramidase. The enhancement of lipoteichoic acid production by fructose and sorbital in batch cultures was not identical in representative strains of S. mutans serotype c, nor was the effect of fructose found uniformly in representative strains of the different S. mutans serotypes.